pRK5 bovine GRK5
(Plasmid #14690)

• Depositing Lab: Robert Lefkowitz
• Publication: Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-13.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GRK5
• Alt name: G protein-coupled receptor kinase 5
• Alt name: GPRK5
• Species: B. taurus (bovine)
• Insert Size (bp): 1900
• Entrez Gene: GRK5 (a.k.a. GPRK5)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The cDNA encoding bovine GRK5 was modified to remove all 3' and 5' untranslated regions. Using standard polymerase chain reaction techniques, a new 5' end containing EcoRI, BglII, and Kozak consensus sequences was added before the start codon, and a new 3' end containing an XbaI site was inserted immediately after the translational stop codon. The amplified fragment was inserted into the EcoRI/XbaI sites of pRK5.