Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32689||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameG protein-coupled receptor kinase 3
SpeciesB. taurus (bovine)
Entrez GeneGRK3 (a.k.a. ADRBK2, BARK2)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site None (not destroyed)
- 3′ cloning site None (not destroyed)
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-GRK3 was a gift from Robert Lefkowitz (Addgene plasmid # 32689 ; http://n2t.net/addgene:32689 ; RRID:Addgene_32689)
For your References section:Phosphatidylinositol 4,5-bisphosphate (PIP2)-enhanced G protein-coupled receptor kinase (GRK) activity. Location, structure, and regulation of the PIP2 binding site distinguishes the GRK subfamilies. Pitcher JA, Fredericks ZL, Stone WC, Premont RT, Stoffel RH, Koch WJ, Lefkowitz RJ. J Biol Chem. 1996 Oct 4. 271(40):24907-13. 10.1074/jbc.271.40.24907 PubMed 8798768