Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32690||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4754
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1800
Entrez GeneGRK4 (a.k.a. GPRK2L, GPRK4, GRK4a, IT11)
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Contains the longest splice variant of GRKa. The insert does not contain the 3'UTR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5-hGRK4 was a gift from Robert Lefkowitz (Addgene plasmid # 32690 ; http://n2t.net/addgene:32690 ; RRID:Addgene_32690)
For your References section:Characterization of the G protein-coupled receptor kinase GRK4. Identification of four splice variants. Premont RT, Macrae AD, Stoffel RH, Chung N, Pitcher JA, Ambrose C, Inglese J, MacDonald ME, Lefkowitz RJ. J Biol Chem. 1996 Mar 15;271(11):6403-10. 10.1074/jbc.271.11.6403 PubMed 8626439