XE69 Xenopus Beta-catenin in GFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16839||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeXenopus Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namebeta-catenin GFP
Alt namebeta catenin
SpeciesX. laevis (frog)
Insert Size (bp)2300
Entrez Genectnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Cla1 (unknown if destroyed)
- 3′ cloning site Cla1 (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
bGFP of Hiem et al., in Cla I site, with stop codon at 3' end. No myc epitope.
Linearize with Not 1 and transcribe with SP6.
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE69 Xenopus Beta-catenin in GFP was a gift from Randall Moon (Addgene plasmid # 16839 ; http://n2t.net/addgene:16839 ; RRID:Addgene_16839)
Map uploaded by the depositor.