XE70 NLS Beta-catenin GFP in CS2+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16838||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameNLS beta-catenin GFP
Alt nameBeta catenin
SpeciesX. laevis (frog)
Entrez Genectnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
NLS of SV40.
Linearize with Not 1 and transcribe with SP6.
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE70 NLS Beta-catenin GFP in CS2+ was a gift from Randall Moon (Addgene plasmid # 16838 ; http://n2t.net/addgene:16838 ; RRID:Addgene_16838)
Map uploaded by the depositor.