|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19887||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5421
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameEukaryotic translation initiation factor 2C, 1
Alt nameEIF2C1; Q99; AGO1; EIF2C; GERP95; DKFZp686M13167
SpeciesH. sapiens (human)
Insert Size (bp)2574
Entrez GeneAGO1 (a.k.a. EIF2C, EIF2C1, GERP95, Q99, hAgo1)
/ Fusion Protein
- FLAG/HA (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFRT/FLAG/HA-DEST EIF2C1 was a gift from Thomas Tuschl (Addgene plasmid # 19887 ; http://n2t.net/addgene:19887 ; RRID:Addgene_19887)
For your References section:Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T. RNA. 2008 Oct 31. ():. 10.1261/rna.1351608 PubMed 18978028