159,238 results

1. M13KO7-ΔPS-ΔgeneIII

   Plasmid

   #134351

   Purpose

   Helper phage for M13 phage production

   Depositor

   Mark Isalan

   Article

   Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

   Insert

   Helper phage gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-Flpo-3xFLAG

   Plasmid

   #173047

   Purpose

   AAV expression of Flpo-3xFLAG by hSyn1 promoter

   Depositor

   Kenji Mizuseki

   Article

   Kitanishi et al Commun Biol. 2022 Feb 21;5(1):149. doi:

   Insert

   Flpo
   

   Use

   AAV

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSNRW-Z5-Nluc

   Plasmid

   #174487

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z5-Nluc
   

   Use

   Tags

   6x Histidine tag (N terminal on insert)

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF6.mCherry-TSG101

   Plasmid

   #38318

   Depositor

   Quan Lu

   Article

   Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   TSG101 (TSG101 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIX403-hNOS2

   Plasmid

   #110800

   Purpose

   tet-inducible NOS2 expression

   Depositor

   Edward Morgan

   Article

   Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

   Insert

   NOS2 (NOS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEG03

   Plasmid

   #154138

   Purpose

   This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

   Depositor

   Kang Zhou

   Article

   Panda et al Biochemical Engineering Journal

   Insert

   fucO, aldA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P_gyrA

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-SapIR

   Plasmid

   #165537

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   SapI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. meGFP-hTRAK1

   Plasmid

   #188664

   Purpose

   human TRAK1 with N-terminal meGFP tag

   Depositor

   Thomas Schwarz

   Insert

   OIP106 (TRAK1 Human)
   

   Use

   Tags

   meGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTLR1

   Plasmid

   #13080

   Depositor

   Ruslan Medzhitov

   Insert

   TLR1 (Tlr1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

10. EF1a_KLF15_P2A_Hygro_Barcode

    Plasmid

    #120501

    Purpose

    Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF15 (KLF15 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTurquoise2-pBAD

    Plasmid

    #54844

    Purpose

    Localization: Protein Expression Vector , Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    6x His, T7 tag, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Lenti-dCas9-TurboID-blast

    Plasmid

    #222599

    Purpose

    Express fussed dCas9-TurboID for proximity labeling

    Depositor

    Yarui Diao

    Article

    Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

    Insert

    dCas9-TurboID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1α

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSA351_SCP1_intron_eGFP

    Plasmid

    #206906

    Purpose

    Lentiviral eGFP reporter vector with SCP1 promoter.

    Depositor

    Stein Aerts

    Article

    Taskiran et al Nature. 2023 Dec 12. doi: 10.1038/s41586

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SCP1

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS_TXS

    Plasmid

    #163839

    Purpose

    iPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthase

    Depositor

    Jerome Fox

    Insert

    taxadiene synthase (61-862)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti NL

    Plasmid

    #113450

    Purpose

    Lentiviral NanoLuc control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    cmyc

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGLOW31

    Plasmid

    #172698

    Purpose

    C. elegans mScarlet co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mScarlet
    

    Use

    Tags

    mScarlet

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available since

    Aug. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. HTR1A-Tango

    Plasmid

    #66404

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1A (HTR1A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mNeonGreen-Rab7

    Plasmid

    #129603

    Purpose

    In vivo visualization of Rab7 (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Rab7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 Mfn2-HA

    Plasmid

    #139192

    Purpose

    Expresses C-terminal HA tagged wt human Mfn2 in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFPC2-Gephyrin P1

    Plasmid

    #68815

    Purpose

    expression of fluorescent tagged Geph in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyri…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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