164,223 results

1. pCW-LIC

   Plasmid

   #26098

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   Three consecutive Taq promoters, 2 active

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. yTREX

   Plasmid

   #177306

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

   Depositor

   Karl-Erich Jaeger

   Article

   Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CycK-GFP (1-270AA) in Cilantro (Codon optimized)

   Plasmid

   #169930

   Purpose

   Overexpression of 1-270AA CycK-GFP fusion

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Insert

   CCNK (CCNK Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDB_347

   Plasmid

   #231114

   Purpose

   CBE activity-based selection cloning vector

   Depositor

   John Doench, David Root

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ230 (LbCpf1)

   Plasmid

   #86210

   Purpose

   LbCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   LbCpf1
   

   Use

   CRISPR; Gateway compatible lbcpf1 entry clone

   Tags

   5' and 3' NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PT3.5/CMV-EGFRvIII

   Plasmid

   #20280

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   EGFRvII (EGFR Human)
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   See depositor comments below.

   Promoter

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS

   Plasmid

   #238243

   Purpose

   For integration of mCherry_P2AT2A_GFP-nb-2xKS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. spNW80

   Plasmid

   #173487

   Purpose

   Expresses spNW80 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW80
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSuper-Retro-Puro-Drp1-shRNA

   Plasmid

   #99385

   Purpose

   Expresses shRNA against human Drp1 from puromycin resistance retroviral vector

   Depositor

   David Kashatus

   Article

   Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

   Insert

   Dynamin-1-like protein (DNM1L Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   H1

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

    Plasmid

    #207862

    Purpose

    AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS U3-hTR-500

    Plasmid

    #28170

    Depositor

    Kathleen Collins

    Article

    Fu et al Mol Cell. 2003 May . 11(5):1361-72.

    Insert

    U3-hTR-500 (TERC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1C

    Plasmid

    #139470

    Purpose

    Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

    Depositor

    David Cobrinik

    Article

    Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

    Insert

    stuffer
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-NLS-AcrIIA4

    Plasmid

    #113037

    Purpose

    Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SV40 NLS-AcrIIA4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV8R-12 Capsid

    Plasmid

    #213968

    Purpose

    AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-R1R4

    Plasmid

    #105110

    Purpose

    create your own 2in1 constructs

    Depositor

    Christopher Grefen

    Article

    Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MTS-mCherry-GFP1-10-Hyg-N1

    Plasmid

    #91957

    Purpose

    Expresses mCherry-GFP1-10 in mammalian cell mitochondria

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    MTS-mCherry-GFP1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-DDX4N-mCh-Cry2WT

    Plasmid

    #101225

    Purpose

    DDX4(1-236) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    DDX4(1-236)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-FGFR3

    Plasmid

    #116742

    Purpose

    Lentiviral expression of FGFR3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2545

    Plasmid

    #144038

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CEBPB (CEBPB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AbVec2.0-mIghg2b

    Plasmid

    #127155

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

    Depositor

    Hedda Wardemann

    Article

    Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

    Insert

    Ighg2b (Ighg2b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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