|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38251||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
- Backbone size (bp) 6126
Modifications to backboneVibrio cholerae MARTX toxin (rtxA, vc1451) cysteine protease domain (CPD) (amino acids 3440-3650) cloned into SalI and XhoI sites in backbone to create fusions with target protein of interest
Vector typeBacterial Expression
/ Fusion Proteins
- Vibrio cholerae MARTX toxin cysteine protease domain (CPD) (C terminal on backbone)
- 6xHis (C terminal on backbone)
Growth in Bacteria
Copy numberLow Copy
- Promoter T7
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
For protein purification, target protein of interest should be cloned into NdeI and SalI sites to create fusion with Vibrio cholerae MARTX toxin cysteine protease domain (CPD)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET22b-CPDSalI was a gift from Matthew Bogyo & Aimee Shen (Addgene plasmid # 38251 ; http://n2t.net/addgene:38251 ; RRID:Addgene_38251)
For your References section:Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag. Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, Garcia KC, Bogyo M. PLoS One. 2009 Dec 2;4(12):e8119. 10.1371/journal.pone.0008119 PubMed 19956581