163,517 results

1. pB80-hKif5b(1-560)rigor-2xmNeongreen

   Plasmid

   #174649

   Purpose

   Live-cell marker for stable microtubules

   Depositor

   Lukas Kapitein

   Article

   Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

   Insert

   hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
   

   Use

   Tags

   mNeongreen-mNeongreen

   Expression

   Mammalian

   Mutation

   rigor mutation G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

   Plasmid

   #233628

   Purpose

   Expression of bicistronic reporter with TOP motif

   Depositor

   Shintaro Iwasaki

   Article

   Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

   Insert

   Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDM042

   Plasmid

   #216812

   Purpose

   Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mScarlet3
   

   Use

   Tags

   VinnyLinker

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE3g-mNeon-WPRE-pA

   Plasmid

   #192064

   Purpose

   mNeonGreen expression with TRE3g promoter

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3g

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-yomTagBFP2-Kan

   Plasmid

   #44899

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomTagBFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_smFP V5

   Plasmid

   #59758

   Purpose

   encodes multiple copies of an epitope tag V5

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _V5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-CreEGFP

   Plasmid

   #165140

   Purpose

   Expression of Cre recombinase fused with EGFP in C-terminal

   Depositor

   David Threadgill, Daekee Lee

   Insert

   Cre recombinase
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CAG-FLInChR-mVenus

   Plasmid

   #119298

   Purpose

   FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

   Depositor

   Alla Karpova

   Article

   Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

   Insert

   Nx1BTM-FCS-ChR2ET/TC-mVenus
   

   Use

   AAV

   Tags

   mVenus

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEmerald-CD36-C-10

   Plasmid

   #54030

   Purpose

   Localization: Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   CD36 (CD36 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pCS6-YFP-SMASh

    Plasmid

    #68853

    Purpose

    Expresses SMASh tag fused to YFP at C terminus in mammalian cells

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    YFP-SMASh
    

    Use

    Tags

    SMASh tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. co luxA pcDNA3.1(+)

    Plasmid

    #129085

    Purpose

    Expression of luxA in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co luxA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. co luxB pcDNA3.1(+)

    Plasmid

    #129086

    Purpose

    Expression of luxB in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co luxB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. IDG_TMEM63B_OE_1

    Plasmid

    #161682

    Purpose

    Overexpresses TMEM63B

    Depositor

    Mike McManus

    Insert

    TMEM63B (TMEM63B Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ-5S-F30-Mango IV

    Plasmid

    #127584

    Purpose

    Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

    Depositor

    David Rueda

    Article

    Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

    Insert

    5S rRNA with F30 folding scaffold and Mango IV tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified U6

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-C3-PLK4-3xFLAG

    Plasmid

    #69837

    Purpose

    This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-mhYFP

    Plasmid

    #186532

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    araB

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGM234

    Plasmid

    #220177

    Purpose

    Expresses exogenous wild-type NAC complex members (NACA and BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T-1_RAF1 (51-131)

    Plasmid

    #119218

    Purpose

    purification of GST-RAF1 RBD from E. coli

    Depositor

    Helen Morrison

    Article

    Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

    Insert

    RAF1 aa 51-131 (RAF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCS-YFP-GW

    Plasmid

    #209361

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, with YFP tag

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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