161,641 results

1. pCAT077

   Plasmid

   #180511

   Purpose

   Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   PlmCasX
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro U6 sgRNA BfuAI stuffer

   Plasmid

   #50920

   Purpose

   U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-FLAG-Claspin

   Plasmid

   #12659

   Depositor

   Michele Pagano

   Article

   Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

   Insert

   Claspin (CLSPN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

   Plasmid

   #84480

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1-sfGFP

   Plasmid

   #102634

   Purpose

   In vitro expression of sfGFP from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   sfGFP
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLF3FShC

   Plasmid

   #153083

   Purpose

   RMCE integration vector with self excising cassette

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-VHL-pRc/CMV

   Plasmid

   #19999

   Depositor

   William Kaelin

   Article

   Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. T7-LacO-LgBiT

   Plasmid

   #216779

   Purpose

   Expresses LgBiT in bacteria

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   LgBiT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-TRIF-CFP

   Plasmid

   #13644

   Depositor

   Doug Golenbock

   Insert

   TRIF (TICAM1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. Halo-Sec31

    Plasmid

    #166895

    Purpose

    mammalian expression of Sec31 tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    Sec31
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS513 EF1alpha-cre

    Plasmid

    #11918

    Purpose

    Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ150

    Plasmid

    #69301

    Purpose

    Gateway entry vector with pcoCas9 (Plant codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9 (plant codon-optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-FOXI1

    Plasmid

    #153126

    Purpose

    Expression of Flag tagged human FOXI1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXI1 (FOXI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCnCre

    Plasmid

    #232907

    Purpose

    Electroporation vector for expression of Cre recombinase in Cuprividus necator

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    Cre recombinase (cre Synthetic)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ptac-cymO

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pI3_EGFP

    Plasmid

    #239335

    Purpose

    Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmScarlet3_I3

    Plasmid

    #239342

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. fabp10a:ER-CreER; cryaa:mCerulean

    Plasmid

    #230077

    Purpose

    Expression of ERCreER in zebrafish hepatocytes.

    Depositor

    Sumeet Pal Singh

    Article

    Hovhannisyan et al bioRxiv 2025.05.23.655316

    Insert

    ERT2-Cre-ERT2
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    fabp10a

    Available since

    May 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV hSyn INTRON mCherry-Jph3 WPRE

    Plasmid

    #236237

    Purpose

    AAV expression of human Junctophilin3 N-terminally tagged with mCherry

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    Junctophilin 3 (JPH3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMY.PRC2

    Plasmid

    #234582

    Purpose

    Expression of ProtA-EZH2, SUZ12, EED and RBBP4

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Insert

    EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pColdI-DDX3_helicase_core

    Plasmid

    #233618

    Purpose

    Expression of His-tagged helicase core region of DDX3X in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core WT (DDX3X Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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