177,821 results

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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Publication

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Promoter

CBA

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-hGPR56-IRES-GFP

Plasmid

#52297

Purpose

Expresses human GPR56 and GFP in mammalian cells.

Depositor

Christopher A Walsh

Publication

Bae et al Science. 2014 Feb 14;343(6172):764-8. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human GPR56 coding sequence (ADGRG1 Human)

Expression

Mammalian

Promoter

CAG promoter

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

Plasmid

#50470

Purpose

Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter

Depositor

Bryan Roth

Insert

hM3D(Gq)-IRES-mCitrine (CHRM3 Human)

Use

AAV

Tags

HA

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPR - EGFP sgRNA 3

Plasmid

#51762

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-p65AD-GBP6

Plasmid

#49440

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GBP6-p65 transactivation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-GBP1-10gly-Gal4DBD

Plasmid

#49438

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GBP1-Gal4 DNA binding domain fusion protein

Expression

Mammalian

Mutation

C98S (to increase protein solubility), Q3D

Promoter

CAG

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pICH47742

Plasmid

#48001

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

11 Articles

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5HT6-G-GECO1.0

Plasmid

#47499

Purpose

Fluorescent reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Publication

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)

Tags

G-GECO1.0

Expression

Mammalian

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTALYM3-Telo1

Plasmid

#47883

Purpose

TALE-mClover against telomere sequence

Depositor

Maria-Elena Torres-Padilla

Publication

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

TALE-mClover

Use

TALEN

Tags

mClover

Expression

Mammalian

Promoter

CAG

Available since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pKIKOrbsARCm

Plasmid

#46765

Purpose

E.coli genomic integration rbsAR locus

Depositor

Lars Nielsen, Claudia Vickers

Publication

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pMM2-hPMCA1b

Plasmid

#47758

Purpose

mammalian expression of human PMCA1b

Depositor

John Penniston, Emanuel Strehler

Publication

Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PMCA1 (ATP2B1 Human)

Expression

Mammalian

Promoter

SV40

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNMT

Plasmid

#42578

Depositor

James Hurley

Publication

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

N-myristoyl tranferase (NMT1 Budding Yeast)

Expression

Bacterial

Mutation

two silent mutations T690C and T942C

Promoter

T7

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pDZ274 (pLEU MET25pro MCP-2x-yeGFP)

Plasmid

#45929

Depositor

Robert Singer, Daniel Zenklusen

Publication

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

MCP-2x-yeGFP

Tags

2x-yeGFP

Expression

Yeast

Promoter

MET25

Available since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pTK168_mCherry-H2B

Plasmid

#46363

Purpose

visualizes chromosomes

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

H2B (H2BC11 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

D26G, V119I and S125D

Promoter

pBABE

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEF-I-GFP GX

Plasmid

#45443

Depositor

John Brigande

Publication

Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

Type

Empty backbone

Tags

IRES EGFP

Expression

Mammalian

Promoter

human EF1α

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
IL2R/hE-cadherin-cytotail

Plasmid

#45773

Depositor

Barry Gumbiner

Publication

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)

Expression

Mammalian

Mutation

Interleukin-2 receptor α subunit extracellular an…

Promoter

CMV

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSMT3-M

Plasmid

#26589

Depositor

Tanya Parish

Publication

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

n/a

Use

Shuttle vector for mycobacteria

Available since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Publication

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(-) MID51 4xMyc Hisx6

Plasmid

#44598

Depositor

David Chan

Publication

Loson et al Mol Biol Cell. 2013 Jan 2.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MiD51 (Mief1 Mouse)

Tags

4xMyc and 6xHis

Expression

Mammalian

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTEF2

Plasmid

#43971

Depositor

Hal Alper

Publication

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TEF

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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