161,439 results

1. pFX-EGFP-EEA1

   Plasmid

   #174451

   Purpose

   Express EGFP with early endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with early endosome-targeting sequence
   

   Use

   Tags

   early endosomal antigen 1 (EEA1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET16b-His-ArgRS

   Plasmid

   #124104

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Argin+I4 ligase (argS Escherichia coli)
   

   Use

   Tags

   10x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30-His-AsnRS

   Plasmid

   #124105

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Asparaginyl-tRNA ligase (asnS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-FRT/T0-Flag-PALB2

   Plasmid

   #71114

   Purpose

   mammalian expession of FLAG-PALB2

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   PALB2 (PALB2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pWPI-SLC7A11-HA

   Plasmid

   #201643

   Purpose

   Over-expression of HA-tagged human SLC7A11

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETMCSI-T4P_Y320A

   Plasmid

   #122522

   Purpose

   Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

   Depositor

   Gottfried Otting

   Article

   Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

   Insert

   gp43 - DNA polymerase (43 Escherichia virus T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   tyrosine 320 changed to alanine

   Promoter

   T7 promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEGFP-30nmSAH-DmrA

   Plasmid

   #61680

   Purpose

   GFP-labeled SAH scaffold with DmrA attachment site

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   mEGFP-30nmSAH-DmrA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-PBZ-mRuby2

   Plasmid

   #110650

   Purpose

   expresses a PAR-binding (PBZ) domain fused to mRuby2

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNTI194

   Plasmid

   #84266

   Purpose

   Expresses Avi-tagged RPL1 and humanized birA

   Depositor

   Nicholas Ingolia

   Article

   Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

   Inserts

   RPL1 (RPL10A (*see below), Human)
   humanized birA
   

   Use

   Tags

   Avi tag, Flag, Gly/Ser linker, and TEV site

   Expression

   Mammalian

   Mutation

   R33A, A34E (*see below)

   Promoter

   CMV and P2A peptide

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. HLA_DRB1_04_01

    Plasmid

    #165013

    Purpose

    Expression of HLA-DRB1*04:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-DRB1*04:01 (HLA-DRB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-EGFP-Tau AP P301L

    Plasmid

    #46906

    Purpose

    expresses EGFP tagged Tau AP P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau AP P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L AND all 14 S/P or T/P amino acid residues (…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLSSmKate1-N1

    Plasmid

    #31866

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSS-mKate1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. miRFP670-TRF1

    Plasmid

    #227388

    Purpose

    Label telomere, no adhesion

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Telomeric miRFP (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSPTg.T2FpAXK (#52)

    Plasmid

    #35962

    Depositor

    Thomas Sato

    Article

    Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

    Inserts

    Tie2 promoter
    SV40 pA
    Tie2 enhancer
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. evoBE4-NG

    Plasmid

    #226852

    Purpose

    Expresses evoBE4max-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    evoBE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-nEFCas9

    Plasmid

    #87115

    Purpose

    SpCas9 expression AAV backbone plasmid under control of nEF promoter

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    nEF-Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_RFP

    Plasmid

    #206177

    Purpose

    mammalian expression of RFP

    Depositor

    Christopher Goodnow

    Article

    Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Man1B1-GFP

    Plasmid

    #163646

    Purpose

    Expresses GFP tagged Man1B1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mannosidase alpha class 1B member 1 (MAN1B1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge