165,567 results

1. pcDNA-R-diLACCO1

   Plasmid

   #208028

   Purpose

   Control biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-diLACCO1
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

   Plasmid

   #209293

   Purpose

   mitoBE expression vector, target mtND4 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Brm WT-Flag pShuttle

   Plasmid

   #122105

   Purpose

   Expression of full length Brm in mammalian cells

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   BRM (SMARCA2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-CD28-mCherry

   Plasmid

   #223593

   Purpose

   Lentiviral expression of human CD28 with mCherry tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   mCherry

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMG023 MBP-Axin 384-518-His (miniAxin)

   Plasmid

   #154012

   Purpose

   Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   Axin1 (AXIN1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   381-518 fragment

   Promoter

   Tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPROBE-NT

   Plasmid

   #37818

   Depositor

   Steven Lindow

   Article

   Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   Promotorless gfp reporter gene
   

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-FLAG-HA-hYTHDC1

   Plasmid

   #85167

   Purpose

   Expression of FLAG-HA-tagged human YTHDC1

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Tags

   Flag and Ha

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

   Plasmid

   #175181

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Has Service

   AAV1

   Insert

   pAAV hSyn FLEX iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI Anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Promoter

   human Synapsin

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-opto-mCasp8

   Plasmid

   #208782

   Purpose

   Inducibly expresses mouse caspase8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mCaspase8 Cry2 (Casp8 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF0589

    Plasmid

    #142653

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Frt-hspB3

    Plasmid

    #63094

    Purpose

    Expression of HSPB3 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb3 (HSPB3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid

    #195487

    Purpose

    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C61T, C262V

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EMTB-3XGFP

    Plasmid

    #26741

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    3x EGFP and Myc

    Expression

    Mammalian

    Mutation

    Only contains the microtubule binding-domain of e…

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid

    #236007

    Purpose

    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    D835Y substitution

    Promoter

    CMV

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

    Plasmid

    #211469

    Purpose

    Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ATAD3A (ATAD3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p-eGFP-beta 2 CP

    Plasmid

    #13298

    Depositor

    John Cooper

    Article

    Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

    Insert

    capping protein beta 2 subunit (Capzb Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221_SLC25A25

    Plasmid

    #131992

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A25 (SLC25A25 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMaCTag-P19

    Plasmid

    #124789

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry-mNeonGreen
    

    Use

    Pcr template

    Tags

    mCherry-mNeonGreen

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF0985

    Plasmid

    #142249

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge