161,439 results

1. pFLAG-TNKS-2

   Plasmid

   #34691

   Depositor

   Nai-Wen Chi

   Article

   Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

   Insert

   TNKS2 (TNKS2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0630

   Plasmid

   #142668

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR1I2 (NR1I2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ143

   Plasmid

   #69295

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-KIF2C

   Plasmid

   #205996

   Purpose

   inducible expression of HA-KIF2C

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   KIF2c (KIF2C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pTight

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRN3P-NLS-EGFP-deadBtgh-3NLS

   Plasmid

   #194470

   Purpose

   Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

   Depositor

   Mathieu Boulard

   Article

   Formichetti et al Dev Cell 10 Jan 2024

   Insert

   BtGH84
   

   Use

   For in vitro transcription from t3 promoter after…

   Tags

   SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

   Expression

   Mutation

   Aspartic Acid 242 (in Dennis et al.) changed to A…

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTO149_RFP_CauNEO

   Plasmid

   #177277

   Purpose

   Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   RFP, CauNEO
   

   Use

   Tags

   ENO1-RFP

   Expression

   Bacterial

   Mutation

   All CUG Leucine codons were replaced with the syn…

   Promoter

   AgTEF2

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA GFP10-ZIP-GFP11

   Plasmid

   #182243

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP-11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe.puro.CDK8.flag

   Plasmid

   #19758

   Depositor

   William Hahn

   Article

   Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

   Insert

   cyclin-dependent kinase 8 (CDK8 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189923

    Purpose

    AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Tags

    Expression

    Mutation

    nSaCas9 KKH, TadA ABE8e V106W

    Promoter

    EFS

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSG06-pLac-NRI

    Plasmid

    #102463

    Purpose

    Expresses NRI protein in E. coli. The expression is controlled by a pLac promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    NRI (ntrC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mKO2-SLBP(18-126)

    Plasmid

    #83914

    Purpose

    Fluorescent probe for S/G2 cell cycle transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mKO2-SLBP(18-126) (SLBP Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTrcAgBIS (CO)

    Plasmid

    #35153

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    Bisabolene Synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHCMV_OROV_GP

    Plasmid

    #207297

    Purpose

    Mammalian expression of Oropouche virus GP protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    OROV_GP (M Oropouche virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. iC13 PB-TO-oNgn2-BFP-puro

    Plasmid

    #204733

    Purpose

    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Optimized neurogenin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-V5-SBP-C1-HshnRNPM

    Plasmid

    #64924

    Purpose

    Expresses V5- and SBP-tagged hnRNPM in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    hnRNPM (HNRNPM Human)
    

    Use

    Tags

    V5 and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pALPS vif

    Plasmid

    #101328

    Purpose

    Encodes codon optimized NL4-3 vif

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 vif
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

    Plasmid

    #149722

    Purpose

    Lentiviral expression of crRNA with GFP and a neomycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF1243

    Plasmid

    #143776

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NW11

    Plasmid

    #133443

    Purpose

    Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge