163,445 results

1. pJOP-HTT-HR45Q

   Plasmid

   #92248

   Purpose

   HTT donor DNA with 45Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-CHD7

   Plasmid

   #170141

   Purpose

   Expresses CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRIM3-rfp

   Plasmid

   #74073

   Purpose

   GentR version of pRIM2 carrying rfp

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   rfp
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDDGFP_LEU2D

   Plasmid

   #58352

   Purpose

   Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

   Depositor

   Simon Newstead

   Article

   Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   GFP-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

   Plasmid

   #196192

   Purpose

   human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
   

   Use

   Znf donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Flag-MIB1-IRES-Puro

   Plasmid

   #140240

   Purpose

   Lentiviral vector expressing flag-tagged MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   Flag-MIB1 (MIB1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2746

   Plasmid

   #144351

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HELZ2 (PRIC285 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-NP-B/BRISBANE/60/2008

   Plasmid

   #187921

   Purpose

   Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   Influenza B Nucleoprotein
   

   Use

   Tags

   UNTAGGED

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Delta(Pro-MP)ADAM10-HA

   Plasmid

   #65107

   Purpose

   Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   Delta(Pro-MP)ADAM10 (ADAM10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted AA 19-455

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pEZYflag

    Plasmid

    #18700

    Purpose

    pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1874

    Plasmid

    #143329

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BBX (BBX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC70197

    Plasmid

    #209458

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-TetON-mEGFP-MYOD1_AroPERFECT

    Plasmid

    #215620

    Purpose

    For integration of MYOD1 AroPERFECT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroPERFECT (MYOD1 Human)
    

    Use

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-NLS-TurboID

    Plasmid

    #163849

    Purpose

    In vitro transcription of nls-TurboID. Parton lab clone JST

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    In vitro transcription of rna

    Tags

    nls

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUGW-H1 empty vector

    Plasmid

    #25870

    Purpose

    3rd generation lentiviral vector

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEmerald-MCAK-C-7

    Plasmid

    #54161

    Purpose

    Localization: Microtubules/Kinesin, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    MCAK (KIF2C Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. AAV-EFS-GFP

    Plasmid

    #170176

    Purpose

    Expresses GFP under the control of EF1a short promoter

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. YIplac211-KAR2-msGFP2-HDEL

    Plasmid

    #160468

    Purpose

    Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    KAR2-msGFP2-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GBX

    Plasmid

    #64123

    Purpose

    a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

    Depositor

    Linzhao Cheng

    Article

    Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

    Insert

    GFP-2A-BCL2L1 (BCL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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