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CRISPRi repression of nonhomologous end-joining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica.
Schwartz C, Frogue K, Ramesh A, Misa J, Wheeldon I
Biotechnol Bioeng. 2017 Aug 19. doi: 10.1002/bit.26404.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
91248pCRISPRi_Mxi1_ylCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
91249pCRISPRi_Mxi1_yl_NHEJCRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR