163,445 results

1. pT3-EF1a-Bmi1

   Plasmid

   #31783

   Depositor

   Xin Chen

   Article

   Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

   Insert

   Bmi1 (BMI1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-miRFP670

   Plasmid

   #91854

   Purpose

   Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

   Depositor

   Ralf Kuehn

   Inserts

   hSpCas9-2A-miRFP670
   BB-guide RNA
   

   Use

   CRISPR

   Tags

   2A-miRFP670 and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh and U6

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGE011

   Plasmid

   #71537

   Purpose

   For CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

   Plasmid

   #132543

   Purpose

   Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

   Depositor

   Bryan Dickinson

   Article

   Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

   Insert

   CIRTS-1: ORF5-TBP6.7-Pin domain-NLS
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   cmv d0

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUA66 theta GFP

   Plasmid

   #105607

   Purpose

   Expresses GFP with no promoter

   Depositor

   Pamela Silver

   Article

   Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   No Promoter

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CT3G-CERP2-MG

   Plasmid

   #175503

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-moxGFP-Blast-H2BC11

   Plasmid

   #207757

   Purpose

   Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEGFP-NR2A

   Plasmid

   #17924

   Depositor

   Stefano Vicini

   Article

   Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

   Insert

   NMDAR2A (Grin2a Rat)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC25A48

   Plasmid

   #131995

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A48 (SLC25A48 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. TFORF2304

    Plasmid

    #142020

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CIITA (CIITA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_BiCHATe27_GCaMP6f

    Plasmid

    #213833

    Purpose

    AAV construct expressing GCaMP6f driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_GCaMP6f

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK1200

    Plasmid

    #84219

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL50010

    Plasmid

    #50310

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3273

    Plasmid

    #144749

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BBX (BBX Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVi U6_gRNA CMV_SadCas9-KRAB

    Plasmid

    #214609

    Purpose

    All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

    Depositor

    Ralf Gilsbach

    Article

    Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

    Inserts

    gRNA
    SadCas9
    

    Use

    AAV

    Tags

    NP NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CMV and U6

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CamKII-LGI1-pHluorin

    Plasmid

    #185537

    Purpose

    Expresses LGI1-pHluorin under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    LGI1 (Lgi1 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid

    #181751

    Purpose

    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLVgag-3xNES-ABE8e
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1436 pcDNA3 Flag HA

    Plasmid

    #10792

    Purpose

    pcDNA3 vector with added Flag, HA tags

    Depositor

    William Sellers

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv2_mSTING_gRNA_1

    Plasmid

    #196625

    Purpose

    Knock-out STING in murine cells, gRNA 1.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING gRNA 1 (Sting1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-KDM4A

    Plasmid

    #188233

    Purpose

    Express human KDM4A in mammalian cells

    Depositor

    Young Tang

    Article

    Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

    Insert

    Lysine-specific demethylase 4A (KDM4A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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