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CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.
Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S
Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73228pNICKclos2.0Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
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73639pNICKclos1.0Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824
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73640pdCASclosTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
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