159,238 results

1. pLEX307 ASC

   Plasmid

   #163977

   Purpose

   Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

   Depositor

   Daniel Bachovchin

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   ASC (PYCARD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

   Plasmid

   #234846

   Purpose

   3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

   Depositor

   Malin Parmar

   Article

   Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IP-EGFP-PA-BAX(ER)

   Plasmid

   #228941

   Purpose

   Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IP-EGFP-LOV2-Cyb5a

   Plasmid

   #233348

   Purpose

   Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus is deleted (Cyb5a 95-134) and N538E

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IZ-SEC61B-mCherry-FRB

   Plasmid

   #233349

   Purpose

   Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   SEC61B (SEC61B Human)
   mCherry
   FRB
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   T2098L mutant of FKBP-rapamycin binding domain of…

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRX-IB-FKBP-HaloTag

   Plasmid

   #233350

   Purpose

   Stable expression of FKBP-Halotag in mammalian cells. FKBP-halotag localizes in the cytosol and binds to the FRB tag in the presence of AP21967 (A/C Heterodimerizer).

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Insert

   FKBP
   

   Use

   Retroviral

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP4_3KB5

   Plasmid

   #234225

   Purpose

   Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3KB5 (TRIM72 Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pRDB_270

   Plasmid

   #231111

   Purpose

   C>T base editor

   Depositor

   John Doench, David Root

   Insert

   TadA-CDd-nCas9-SpG-UGI-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6-sasgRNACMV- mCherry-WPREpA

   Plasmid

   #217015

   Purpose

   S. aureus sgRNAs backbone along with mCherry expression

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4_NFAT-RE-CMVmin-BC0538-luc2

    Plasmid

    #227140

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC05038

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid

    #227332

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SNAP-BAF

    Plasmid

    #227443

    Purpose

    Mammalian expression of SNAP-BAF

    Depositor

    David Pellman

    Article

    Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

    Insert

    Barrier-to-autointegration factor (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AfNitB_pET29b

    Plasmid

    #215401

    Purpose

    Expression construct for AfNitB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    AfNitB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MEK1-E102-I103del-V5

    Plasmid

    #217301

    Purpose

    Lentivirus vector for expression of MEK1 E102-I103deletion

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    E102-I103deletion

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. EXT1- D164A-pLJC2-3XFLAG

    Plasmid

    #215828

    Purpose

    Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

    Plasmid

    #211969

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

    Plasmid

    #211968

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p194-RV-CAG-dio-mScarlet-T2A-Brn2

    Plasmid

    #204741

    Purpose

    Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    Brn2
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPPC005

    Plasmid

    #171140

    Purpose

    For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    XylS-Pm
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJT121_GalL_nCDA1-VQRBE3

    Plasmid

    #145086

    Purpose

    Expressing base editor nCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VQRBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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