165,567 results

1. pAAV2-sgTrp53-sgAtrx-EFS-Cre

   Plasmid

   #189977

   Purpose

   Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

   Depositor

   William Kaelin

   Article

   Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

   Insert

   sgTrp53, sgAtrx, Cre recombinase
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-EFS-foldon-GFP- PCP

   Plasmid

   #194503

   Purpose

   Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

   Depositor

   Chunqing Song

   Article

   Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

   Insert

   foldon-GFP-PCP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1α core promoter

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-MCS

   Plasmid

   #185594

   Purpose

   Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213969

   Purpose

   AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. (SUMO)10

   Plasmid

   #126948

   Purpose

   Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
   

   Expression

   Bacterial

   Promoter

   unknown

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMVTNT-T7-KPNA5

   Plasmid

   #26681

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 5 (KPNA5 Human)
   

   Tags

   T7 (MASMTGGQQMG)

   Expression

   Mammalian

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-OMP25

   Plasmid

   #141150

   Purpose

   Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   OMP25 (Synj2bp Rat)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   OMP25 c terminus from addgene plasmid #69598

   Promoter

   CMV

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-HA-hYTHDC1

   Plasmid

   #85167

   Purpose

   Expression of FLAG-HA-tagged human YTHDC1

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Tags

   Flag and Ha

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GPR68-Tango

   Plasmid

   #66371

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR68 (GPR68 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE/VEGF/IRES/EGFP

    Plasmid

    #206211

    Purpose

    An expression vector of VEGF and EGFP under control of TRE promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    vascular endothelial growth factor (Vegfa Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AiP15079 - pAAV-AiE0127m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5079)

    Plasmid

    #230403

    Purpose

    AiE0127m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-KO-PGK-Puro

    Plasmid

    #227264

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid

    #228253

    Purpose

    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2-CAG-3xNLS-mScarlet

    Plasmid

    #191098

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to human codon usage

    Promoter

    CMV enhancer and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-VP7SA11

    Plasmid

    #89167

    Purpose

    Express Rotavirus SA11 VP7 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP7
    

    Tags

    *see below

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol1-UAS:GtACR1-tdTomato

    Plasmid

    #124235

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    GtACR1-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPRIME-CMV-LNGFR-recipient

    Plasmid

    #11660

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    LNGFR

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCM184-ParB2::eGFP

    Plasmid

    #87252

    Purpose

    Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB2
    

    Tags

    EGFP

    Expression

    Yeast

    Promoter

    pBAD/tetO

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPROBE-NT

    Plasmid

    #37818

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotorless gfp reporter gene
    

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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