161,570 results

1. pT7C-HSP90AB1

   Plasmid

   #177659

   Purpose

   Recombinant protein synthesis of human HSP90AB1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSP90AB1 (HSP90AB1 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT/FLAG/HA-DEST PUM2

   Plasmid

   #40292

   Depositor

   Thomas Tuschl

   Article

   Hafner et al Cell. 2010 Apr 2;141(1):129-41.

   Insert

   Pumilio homolog 2 (PUM2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA24N-ligase-NLS-cTF

   Plasmid

   #87746

   Purpose

   IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and NLS linker - cTF (chimeric transcriptio…

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-SS-345

   Plasmid

   #68785

   Purpose

   R->A Sensor pair 1 with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   C-Terminal Sensor 1 V2 R-->A mutant
   N-Terminal Sensor 1 V2 R-->A mutant
   mCherry
   

   Use

   Tags

   NLS, flag, and nls

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-ccdB-3xHA

   Plasmid

   #214986

   Purpose

   Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag

   Depositor

   Wade Harper

   Type

   Empty backbone

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

   Plasmid

   #87693

   Purpose

   Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1436 pcDNA3 Flag HA

   Plasmid

   #10792

   Purpose

   pcDNA3 vector with added Flag, HA tags

   Depositor

   William Sellers

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

   Plasmid

   #220735

   Purpose

   AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBOB-CAG-SARS-CoV2-Spike-HA

   Plasmid

   #141347

   Purpose

   3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   resynthesized with human codon optimization nucle…

   Promoter

   CAG

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-TO-Vpr-mRuby3-IN

    Plasmid

    #167584

    Purpose

    For producing fluorescently labeled HIV-1; expresses HIV-1 integrase fused to Vpr for packaging into HIV-1 and the fluorophore mRuby3

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    Vpr-mRuby3-IN
    

    Use

    Tags

    Vpr-mRuby3

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-C1-CAMKIIalpha

    Plasmid

    #21226

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIalpha (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0001.

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. LV 1-5

    Plasmid

    #68411

    Purpose

    Lentiviral backbone for use with GMAP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Type

    Empty backbone

    Use

    Lentiviral; Gmap

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208048

    Purpose

    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    BirAopt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BirAopt is human-codon-optimized BirA (PMID 15707…

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. 7TFC

    Plasmid

    #24307

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTol-Promoter:NLS-tdMCP-eGFP

    Plasmid

    #86248

    Purpose

    For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Insert

    beta Actin promoter:NLS-tdMCPeGFP
    

    Use

    Used with transposase to make transient or stable…

    Tags

    NLS-tdMCP-eGFP

    Expression

    Mutation

    Promoter

    Beta Actin

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACYC-Taq

    Plasmid

    #102793

    Purpose

    Expresses Taq DNA polymerase under WT T7 promoter

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    Taq DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NADK

    Plasmid

    #28268

    Depositor

    Cheryl Arrowsmith

    Insert

    NADK:HPC0AC-F12:C216828 (NADK Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains residues 68-426.

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #109048

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Has Service

    AAV9

    Insert

    eGtACR1-ST
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9 TadDE

    Plasmid

    #193837

    Purpose

    Express TadDE (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-DualEditor-Cas9D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge