165,688 results

1. pcDNA3.1 acGFP

   Plasmid

   #128047

   Purpose

   Mammalian expression vector with N terminal AcGFP

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Tags

   AcGFP

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_Cloning Vector BbsI

   Plasmid

   #128433

   Purpose

   Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

   Depositor

   Izuho Hatada

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HCA2-Tango

   Plasmid

   #66396

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HCA2 (HCAR2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-ESR1-Y537N

   Plasmid

   #116373

   Purpose

   Lentiviral expression of ESR1 Y537N

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Lentiviral

   Mutation

   Y537N

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

   Plasmid

   #233080

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1i

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.4_7C11-1 light chain

   Plasmid

   #198252

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
   

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.4_7C11-1 heavy chain

   Plasmid

   #198251

   Purpose

   recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)
   

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-APEX2-NIK3x

   Plasmid

   #129274

   Purpose

   Expression of APEX2 in the nucleolus

   Depositor

   Alice Ting

   Article

   Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

   Insert

   GFP-APEX2-NIK3x
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. luxB:cp157Venus+luxA/pRSETB Duet

   Plasmid

   #197583

   Purpose

   BRET lux luciferase with cp157Venus for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   LuxA LuxBcp157Venus
   

   Use

   E.coli expression

   Tags

   His X 6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_FYN_WT

    Plasmid

    #82211

    Purpose

    Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FYN (FYN Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. epB_UbC_CymRV5-nls-GFP_DEx2

    Plasmid

    #119906

    Purpose

    ePiggyBac mammalian expression plasmid for CymR-GFP fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    CymRV5-nls-GFP
    

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROSA26-CreERT2

    Plasmid

    #149437

    Purpose

    ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRY2-GFP-homer1c

    Plasmid

    #89442

    Purpose

    Localizes CRY2 to excitatory synapses

    Depositor

    Matthew Kennedy

    Article

    Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

    Insert

    CRY2-GFP-homer1c
    

    Expression

    Mammalian

    Mutation

    Contains Full length CRY2

    Promoter

    CAG

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRF-TetR

    Plasmid

    #49374

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    TetR repressor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCh-KIF5B*-strep

    Plasmid

    #120164

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_SLC6A15

    Plasmid

    #131877

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A15 (SLC6A15 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. qTAG-N-Puro-mStayGold-ACTB

    Plasmid

    #227320

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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