159,238 results

1. pcDNA3.1-Tat101(AD8)-Flag

   Plasmid

   #115777

   Purpose

   pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

   Depositor

   Damian Purcell

   Article

   Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

   Insert

   HIV-1 Tat protein (AD8)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

   Plasmid

   #39299

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. PA-mCherry1-Fascin-C-10

   Plasmid

   #57177

   Purpose

   Localization: Cytoskeleton, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Fascin (FSCN1 Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. JD150

   Plasmid

   #229485

   Purpose

   Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E2A, VA RNA I, E4orf6, L4-22k/33k
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENN.AAV.CMVs.TurboRFP.WPRE.RBG

   Plasmid

   #105548

   Purpose

   AAV expression of TurboRFP from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV1 and AAV8

   Insert

   TurboRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1-5'dGFP3'

   Plasmid

   #26425

   Depositor

   Erin Schuman

   Article

   Sutton et al Science. 2004 Jun 25. 304(5679):1979-83.

   Insert

   5'dGFP3'
   

   Use

   Tags

   CamKIIa 3'-UTR, CamKIIa 5'-UTR, and dGF…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pAPM-D4 miR30-TRIM28 ts1

   Plasmid

   #115862

   Purpose

   TRIM28 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   TRIM28 shRNA (TRIM28 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-Flpo-3xFLAG

   Plasmid

   #173047

   Purpose

   AAV expression of Flpo-3xFLAG by hSyn1 promoter

   Depositor

   Kenji Mizuseki

   Article

   Kitanishi et al Commun Biol. 2022 Feb 21;5(1):149. doi:

   Insert

   Flpo
   

   Use

   AAV

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0086

   Plasmid

   #141510

   Purpose

   Lentiviral vector for overexpressing the LIN28B transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LIN28B (LIN28B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH-EF1-GFP

    Plasmid

    #133018

    Purpose

    (Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

    Depositor

    Michael Huen

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-luxOFP

    Plasmid

    #229945

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

    Insert

    luxOFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEG03

    Plasmid

    #154138

    Purpose

    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

    Depositor

    Kang Zhou

    Article

    Panda et al Biochemical Engineering Journal

    Insert

    fucO, aldA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P_gyrA

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_KLF15_P2A_Hygro_Barcode

    Plasmid

    #120501

    Purpose

    Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF15 (KLF15 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. EK0396 BFPmut-stop-t70587 (pEAQ)

    Plasmid

    #191171

    Purpose

    EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    t70587 (plant)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS_TXS

    Plasmid

    #163839

    Purpose

    iPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthase

    Depositor

    Jerome Fox

    Insert

    taxadiene synthase (61-862)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB5050

    Plasmid

    #171126

    Purpose

    A plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

    Insert

    OsTIR1
    

    Use

    Other

    Tags

    Expression

    Mutation

    non applicable

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FASTHDR-Cterm-HaloTag-Puromycin

    Plasmid

    #167208

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MS2-HB

    Plasmid

    #35573

    Depositor

    Marian Waterman

    Article

    Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

    Insert

    MS2 Coat Protein (cp Bacteriophage)
    

    Use

    Retroviral

    Tags

    HTBH

    Expression

    Mammalian

    Mutation

    Insert is a tail-to-head tandem placement of two …

    Promoter

    CMV

    Available since

    May 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-UCHL5

    Plasmid

    #22565

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    UCHL5 (UCHL5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Use

    Tags

    V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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