Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

173,197 results

Showing: 11261 - 11280 of 173197 results

  7. pETM33_Nsp1

    Plasmid
    #156465
    Purpose
    Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156465-plasmid-map-sequence-id-307729

    Enlarge

  9. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

    Enlarge

  12. mTagBFP-Nucleus-7

    Plasmid
    #55265
    Purpose
    Localization: Nuclear Localization, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mTagBFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55265-plasmid-map-sequence-id-475376

    Enlarge

  13. S1PR3-Tango

    Plasmid
    #66498
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    S1PR3 (S1PR3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pSG5 Large T delta 434-444

    Plasmid
    #9054
    Depositor
    William Hahn
    Insert
    large T antigen d434-444 (SV40gp6 simian virus)
    Expression
    Mammalian
    Mutation
    deletion in part of the bipartite p53 binding dom…
    Available Since
    Nov. 3, 2005
    Availability
    Academic Institutions and Nonprofits only
Showing: 11261 - 11280 of 173197 results