pCMV-Neo-Bam APC 1-1941
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16510||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 6600
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)9000
MutationCodon 1941 truncation
Entrez GeneAPC (a.k.a. BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
This truncation was created using site-directed mutagenesis.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-Neo-Bam APC 1-1941 was a gift from Bert Vogelstein (Addgene plasmid # 16510 ; http://n2t.net/addgene:16510 ; RRID:Addgene_16510)
For your References section:Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, Kinzler KW. Science. 1997 Mar 21. 275(5307):1787-90. 10.1126/science.275.5307.1787 PubMed 9065402
Map uploaded by the depositor.