165,688 results

1. pAd/CMV/DAX1-IRES-nEGFP

   Plasmid

   #29752

   Depositor

   Edward McCabe

   Insert

   dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
   

   Tags

   EGFP, IRES, and Nuclear localization sequence

   Expression

   Mammalian

   Mutation

   No mutations; Reporter is nuclear localized EGFP;…

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-mCherry

   Plasmid

   #197420

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mPlum-pBAD

   Plasmid

   #54564

   Purpose

   Localization: Protein Expression Vector , Excitation: 590, Emission: 649

   Depositor

   Michael Davidson, Roger Tsien

   Article

   Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

   Type

   Empty backbone

   Tags

   6xHis and mPlum

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. huCaV2.2 (pSAD442-1)

   Plasmid

   #62574

   Purpose

   Human CaV2.2 expression in mammalian cells

   Depositor

   Diane Lipscombe

   Article

   Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

   Insert

   CACNA1B (CACNA1B Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Ii-OVA.I+II

   Plasmid

   #64596

   Purpose

   Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

   Depositor

   Martin Zenke, Sandra Diebold

   Article

   Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

   Insert

   Ovalbumin
   

   Tags

   Invariant chain

   Expression

   Mammalian

   Mutation

   Only amino acids 242-353 (class I and class II ep…

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFlag-CMV2-Brd4 del ET

   Plasmid

   #21938

   Depositor

   Eric Verdin

   Article

   Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 1-1362 only, ET domain deleted

   Available Since

   Sept. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

   Plasmid

   #165450

   Purpose

   Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9N-IntN
   3x Opn1mw promoter-targeting sgRNAs
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6 and short human rhodopsin promoter

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJG100

   Plasmid

   #138966

   Purpose

   Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   wrmScarlet1-10
   

   Expression

   Worm

   Available Since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51083

    Purpose

    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHel3

    Plasmid

    #102961

    Purpose

    E.coli/H.Pylori shuttle vector

    Depositor

    Rainer Haas

    Article

    Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

    Type

    Empty backbone

    Use

    Shuttle vector

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) Flag-His-ATM kd

    Plasmid

    #31986

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM kd (ATM Human)
    

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    3'UTRf 9 nucl/D2870A N2875k. catalytically …

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1_gRNA

    Plasmid

    #196139

    Purpose

    gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    AAVS1 gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTarget_CAST

    Plasmid

    #127926

    Purpose

    Target plasmid containing PSP1

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    PSP1 target (GGTT PAM)
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pESC-MET

    Plasmid

    #222482

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    FLAG and Myc

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pESC-LYS

    Plasmid

    #222483

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    FLAG and Myc

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF1α-Cre-2A-moxGFP

    Plasmid

    #207744

    Purpose

    Expression of the Cre recombinase linked to a moxGFP fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-moxGFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pMXS-GshF-blasticidin

    Plasmid

    #210313

    Purpose

    Expresses humanized Streptococcus thermophilus GshF in mammalian cells

    Depositor

    Kivanc Birsoy

    Article

    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

    Insert

    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    

    Use

    Retroviral

    Tags

    FLAG peptide

    Mutation

    Codon optimized for expression in human cells

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Human Parkin (1-465)

    Plasmid

    #110758

    Purpose

    Bacterial expression of human Parkin

    Depositor

    David Komander

    Article

    Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

    Insert

    PRKN (PRKN Human)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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