176,221 results

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pPAICS-EGFP

Plasmid

#99108

Purpose

Expresses human PAICS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Publication

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PAICS (PAICS Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Publication

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Expression

Mammalian

Promoter

CAG

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CBh-DIO-EGFP

Plasmid

#87168

Purpose

Expression of eGFP in the presence of Cre

Depositor

Jimok Kim

Publication

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

EGFP

Use

AAV

Tags

None

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCM189-ParB1::mCherry

Plasmid

#87253

Purpose

Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

Depositor

Kerstin Bystricky

Publication

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB1

Tags

mCherry

Expression

Yeast

Promoter

pBAD/tetO

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TRE-mTurquoise2

Plasmid

#99113

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAsCpf1(TYCV)(BB) (pY211)

Plasmid

#89352

Purpose

Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

Depositor

Feng Zhang

Publication

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CBh

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TRE-FLEX-eNpHR-EYFP

Plasmid

#89876

Purpose

To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

eNpHR=EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

TetO

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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CD8a-EGFP

Plasmid

#86051

Purpose

A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

Plasmid

#89508

Purpose

Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

Depositor

Jin-Soo Kim

Publication

Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

Insert

rAPOBEC1-nCas9 (D10A)-UGI-NLS

Tags

UGI and rAPOBEC-XTEN

Expression

Bacterial

Mutation

D10A mutation of Cas9

Promoter

T7

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_NOTCH2_WT

Plasmid

#82292

Purpose

Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NOTCH2 (NOTCH2 Human)

Use

Gateway Cloning

Mutation

C946Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2-GFP-homer1c

Plasmid

#89442

Purpose

Localizes CRY2 to excitatory synapses

Depositor

Matthew Kennedy

Publication

Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

Insert

CRY2-GFP-homer1c

Expression

Mammalian

Mutation

Contains Full length CRY2

Promoter

CAG

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ141-ZmUbi-RZ-As

Plasmid

#86196

Purpose

AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Depositor

Yiping Qi

Publication

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

AsCpf1 gRNA cloning site for ribozyme cleavage

Use

CRISPR

Tags

Hammerhead ribozyme and HDV ribozyme

Expression

Plant

Promoter

Maize ubiquitin 1

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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YFC43

Plasmid

#89488

Purpose

Gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFP

Depositor

Alejandro Ferrando

Publication

Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

Type

Empty backbone

Tags

YFP-C (aa 154-240)

Expression

Plant

Promoter

2x35S

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YFN43

Plasmid

#89487

Purpose

Gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP

Depositor

Alejandro Ferrando

Publication

Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

Type

Empty backbone

Tags

YFP-N (aa 1-154)

Expression

Plant

Promoter

2x35S

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFG4

Plasmid

#87251

Purpose

gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

Depositor

Kerstin Bystricky

Publication

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Hyg

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Publication

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MSCV-(mcs) human CSF1 full length-IRES-Puro

Plasmid

#86797

Purpose

retroviral expression of CSF-1

Depositor

Martine Roussel, Charles Sherr

Publication

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-Puro

Expression

Mammalian

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRH2521

Plasmid

#84380

Purpose

Expression of sgRNA from a imyc promoter (Pimyc)

Depositor

Robert Husson

Publication

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

sgRNA cloning site

Use

CRISPR

Expression

Bacterial

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pENTR221 UBR5

Plasmid

#81062

Purpose

Gateway entry vector encoding full length UBR5

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)

Use

Cloning

Mutation

K503R

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

Plasmid

#80840

Purpose

Modified helper phage

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV

Expression

Bacterial

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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