163,504 results

1. reporter-gT1

   Plasmid

   #47320

   Purpose

   TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   minCMV-dTomato_pA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-N-Flag-NLRP3 1-90

   Plasmid

   #75137

   Purpose

   Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 1-90

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTagBFP2-Annexin-12

   Plasmid

   #55276

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Annexin (A4) (ANXA4 Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLentiCRISPRv2_mSTING_gRNA_1

   Plasmid

   #196625

   Purpose

   Knock-out STING in murine cells, gRNA 1.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING gRNA 1 (Sting1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. UKNP1.16.3

   Plasmid

   #98188

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST14-SpyCatcher002

   Plasmid

   #102827

   Purpose

   Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSP3535

   Plasmid

   #46886

   Purpose

   Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

   Depositor

   Gary Dunny

   Article

   Bryan et al Plasmid. 2000 Sep;44(2):183-90.

   Type

   Empty backbone

   Use

   Nisin-inducible expression; gram-positive bacteri…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PnisA

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEUI(+)

   Plasmid

   #111834

   Purpose

   For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.

   Depositor

   Hyunju Ro

   Article

   Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)

   Insert

   GAL4-minimal VP16-EcR (EcR Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SV40, 10XUAS

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.GFAP.SV40.eGFP.SV40(polyA)

   Plasmid

   #195558

   Purpose

   Expresses eGFP under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   No tags

   Expression

   Mammalian

   Mutation

   Promoter

   short GFAP

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PV-NES-GFP

    Plasmid

    #17301

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-NES-GFP (Pvalb Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157024

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    JAG1 (JAG1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-GST-AGO2

    Plasmid

    #24317

    Depositor

    Carl Novina

    Article

    Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

    Insert

    human AGO2 (AGO2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xmts-mNeonGreen

    Plasmid

    #98876

    Purpose

    In vivo visualization of mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    4xmts
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TGT-His

    Plasmid

    #138201

    Purpose

    Expresses E. Coli TGT with a C-terminal His Tag

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Insert

    E Coli tRNA Guanine Transglycosylase-His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS Omomyc

    Plasmid

    #113164

    Purpose

    Omomyc expression in mammalian cells

    Depositor

    Sergio Nasi

    Article

    Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

    Insert

    Omomyc (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Promoter

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TALED_Left-ND1-E1347A

    Plasmid

    #183895

    Purpose

    Expresses TALED_Left-ND1-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEED-mScarlet3

    Plasmid

    #219640

    Purpose

    Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CT3G-CERP2-MG

    Plasmid

    #175503

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCMV-MokolaG

    Plasmid

    #15811

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    Mokola virus glycoprotein (G) gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3TS-TurboID-EGFP

    Plasmid

    #163846

    Purpose

    In vitro transcription of TurboID-EGFP. Parton lab clone JRS

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    In vitro transcription of rna

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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