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Axin2 probe
(Plasmid #21277)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21277 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    For riboprobe synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Axin2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • Mutation
    cDNA bp 1774 to 2787
  • Entrez Gene
    Axin2 (a.k.a. Axi1, Axil, Conductin)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Tong DNA #94. The DNA is a HinDIII fragment of mouse Axin2 (nucleotides 1-2397) in pBluescript-KS. The antisense and sense Axin2 riboprobes were made by linearizing this plasmid with NotI or SalI and transcribing with T3 or T7 RNA polymerase, respectively. List of DNAs says " ~2 kb DIII fragment of Axin2 (1-2397) in pBS-KS”.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Axin2 probe was a gift from Frank Costantini (Addgene plasmid # 21277 ; http://n2t.net/addgene:21277 ; RRID:Addgene_21277)
  • For your References section:

    Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway. Jho EH, Zhang T, Domon C, Joo CK, Freund JN, Costantini F. Mol Cell Biol. 2002 Feb . 22(4):1172-83. 10.1128/MCB.22.4.1172-1183.2002 PubMed 11809808