Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21277||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS
- Backbone size w/o insert (bp) 3000
Vector typeFor riboprobe synthesis
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2000
MutationcDNA bp 1774 to 2787
Entrez GeneAxin2 (a.k.a. Axi1, Axil, Conductin)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Tong DNA #94. The DNA is a HinDIII fragment of mouse Axin2 (nucleotides 1-2397) in pBluescript-KS. The antisense and sense Axin2 riboprobes were made by linearizing this plasmid with NotI or SalI and transcribing with T3 or T7 RNA polymerase, respectively. List of DNAs says " ~2 kb DIII fragment of Axin2 (1-2397) in pBS-KS”.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Axin2 probe was a gift from Frank Costantini (Addgene plasmid # 21277 ; http://n2t.net/addgene:21277 ; RRID:Addgene_21277)
For your References section:Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway. Jho EH, Zhang T, Domon C, Joo CK, Freund JN, Costantini F. Mol Cell Biol. 2002 Feb . 22(4):1172-83. 10.1128/MCB.22.4.1172-1183.2002 PubMed 11809808