Axin2 probe
(Plasmid #21277)

• Depositing Lab: Frank Costantini
• Publication: Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Backbone

• Vector backbone: pBluescript II KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: For riboprobe synthesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Axin2
• Species: M. musculus (mouse)
• Insert Size (bp): 2000
• Mutation: cDNA bp 1774 to 2787
• Entrez Gene: Axin2 (a.k.a. A, Axi1, Axil, Condu, Conductin)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tong DNA #94. The DNA is a HinDIII fragment of mouse Axin2 (nucleotides 1-2397) in pBluescript-KS. The antisense and sense Axin2 riboprobes were made by linearizing this plasmid with NotI or SalI and transcribing with T3 or T7 RNA polymerase, respectively. List of DNAs says " ~2 kb DIII fragment of Axin2 (1-2397) in pBS-KS”.