159,237 results

1. pGL3-Basic-8x-gRNA-eGFP

   Plasmid

   #60718

   Purpose

   eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ymScarlet

   Plasmid

   #111917

   Purpose

   yeast optimized mScarlet

   Depositor

   Patrick Lajoie

   Article

   Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

   Insert

   ymScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

   Plasmid

   #106309

   Purpose

   The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

   Depositor

   James Zhe Liu

   Article

   Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

   Insert

   6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
   

   Use

   Tags

   6xflag-3xHaloTag(MtoL)

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GPR37-DuET

   Plasmid

   #213282

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR37 (GPR37 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CL20_mEGFP_NUP98_HOXD13

   Plasmid

   #205871

   Purpose

   Express mEGFP-tagged fusion protein, NUP98_HOXD13 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NUP98_HOXD13 (NUP98, HOXD13 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAWP78-PVCpnf_pvc13-CD11bDARPin

   Plasmid

   #198295

   Purpose

   Produces PVCs retargeted with anti-CD11b DARPin F7

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-CD11bDARPin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ITGA5-bio-His

   Plasmid

   #51909

   Purpose

   Expresses full-length Integrin alpha-5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ITGA5 (ITGA5 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-AcGFP1-ORP9-PH

   Plasmid

   #214266

   Purpose

   Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   AcGFP1-ORP9(1-111) (Osbpl9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA6-N-Flag-SREBP1

   Plasmid

   #134283

   Purpose

   Expresses Flag-tagged SREBP1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP1 (Srebf1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP1 isoform a precursor

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-NRF1

    Plasmid

    #34707

    Depositor

    Raymond Deshaies

    Article

    Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

    Insert

    NRF1 (NFE2L1 Human)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pEYFP-C1-Drp1

    Plasmid

    #45160

    Depositor

    Richard Youle

    Article

    Frank et al Dev Cell. 2001 Oct;1(4):515-25.

    Insert

    Drp1 (DNM1L Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Vamp3-pHluorin

    Plasmid

    #190152

    Purpose

    Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp3 (Vamp3 Rat)
    

    Use

    AAV

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVET-HA-K-RasG12V-IRES-GFP

    Plasmid

    #107140

    Purpose

    A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Insert

    HA-tagged Kras4B(G12V) and GFP (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1alpha

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCF806-shABC2

    Plasmid

    #186714

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. myrAkt delta4-129

    Plasmid

    #10841

    Depositor

    Richard Roth

    Article

    Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

    Insert

    Akt (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Deletion of PH domain (aa4-129)

    Promoter

    SV40

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLPC-NFLAG TRF1

    Plasmid

    #16058

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    aa2-439

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pSYN-PC7-006

    Plasmid

    #42502

    Depositor

    Robert Peters

    Article

    Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

    Insert

    PC7-SOL CDS (PCSK7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMP11

    Plasmid

    #215559

    Purpose

    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    Cas9
    gRNA targeting pBR322 origin of replication
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105 and tet

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-Elongin B-pcDNA3.1(+)-Zeo

    Plasmid

    #20000

    Depositor

    William Kaelin

    Article

    Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

    Insert

    transcription elongation factor B (ELOB Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLIK_hygro_BirA_NRF2

    Plasmid

    #136539

    Purpose

    Lentiviral expression vector for an inducible BirA-NRF2

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA-NRF2 (NFE2L2 Human)
    

    Use

    Lentiviral; Destination vector for gateway cloning

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge