Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #188521)


Item Catalog # Description Quantity Price (USD)
Plasmid 188521 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Eric Kowarz Lab
  • Backbone size w/o insert (bp) 6350
  • Total vector size (bp) 6948
  • Vector type
    Mammalian Expression ; Transposon mediated gene integration
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Synthetic; Aquifex aeolicus
  • Insert Size (bp)
  • Promoter EF1a/RPBSA
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI/NcoI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBbi_RP_MicroID2-HA was a gift from James Londino (Addgene plasmid # 188521 ; ; RRID:Addgene_188521)
  • For your References section:

    MicroID2: A Novel Biotin Ligase Enables Rapid Proximity Dependent Proteomics. Johnson BS, Chafin L, Farkas D, Adair J, Elhance A, Farkas L, Bednash JS, Londino JD. Mol Cell Proteomics. 2022 Jun 7:100256. doi: 10.1016/j.mcpro.2022.100256. 10.1016/j.mcpro.2022.100256 PubMed 35688383