AAV2/9 REP-AAP-ΔCAP
(Plasmid
#205991)
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PurposeRep and AAP protein expression for AAV CREATE libraries
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonerecombinant AAV2/9
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAAV9 Rep and AAP proteins for CREATE libraries
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This work was supported by funding from:
NIH BRAIN Armamentarium UF1MH128336
Michael J Fox Foundation for Parkinson's Research ASAP-020495
Additional information may be found in Ravindra Kumar, Sripriya et al. “Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types.” Nature methods vol. 17,5 (2020): 541-550. doi:10.1038/s41592-020-0799-7
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV2/9 REP-AAP-ΔCAP was a gift from Viviana Gradinaru (Addgene plasmid # 205991 ; http://n2t.net/addgene:205991 ; RRID:Addgene_205991) -
For your References section:
Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Chuapoco MR, Flytzanis NC, Goeden N, Christopher Octeau J, Roxas KM, Chan KY, Scherrer J, Winchester J, Blackburn RJ, Campos LJ, Man KNM, Sun J, Chen X, Lefevre A, Singh VP, Arokiaraj CM, Shay TF, Vendemiatti J, Jang MJ, Mich JK, Bishaw Y, Gore BB, Omstead V, Taskin N, Weed N, Levi BP, Ting JT, Miller CT, Deverman BE, Pickel J, Tian L, Fox AS, Gradinaru V. Nat Nanotechnol. 2023 Jul 10. doi: 10.1038/s41565-023-01419-x. 10.1038/s41565-023-01419-x PubMed 37430038
