159,237 results

1. pet151D-TOPO ALIX Bro1-V (1-702)

   Plasmid

   #80642

   Purpose

   Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648

   Depositor

   Wesley Sundquist

   Article

   McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

   Insert

   ALIX Bro1-V (PDCD6IP Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Residues 1-702

   Promoter

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-GFP-Rim2

   Plasmid

   #170869

   Purpose

   Mammalian expression of His-GFP-Rim2.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)
   

   Use

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR myc-MBP-SspB-iRFP-NLS

   Plasmid

   #223286

   Purpose

   An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   Myc-MBP-SspB-irFP-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-CMV-jGCaMP7b

   Plasmid

   #104484

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7b
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-FGR

   Plasmid

   #23877

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   FGR (FGR Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pET28b-pchG WT

   Plasmid

   #87107

   Purpose

   expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

   Insert

   pchG WT
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Nrf1-Myc pcDNA3.1(+)-C-Myc

   Plasmid

   #169125

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hNrf1 (NFE2L1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-D12L

   Plasmid

   #89161

   Purpose

   Express vaccinia virus D12L in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D12L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promotor

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Mem

   Plasmid

   #55779

   Purpose

   This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   mCherry-Mem
   

   Use

   Tags

   The N-terminal 20 residues of neuromodulin are fu…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTKDP-dhfr

    Plasmid

    #71323

    Purpose

    Donor plasmid - trimethoprim resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.16

    Plasmid

    #212532

    Purpose

    Encodes SARS-CoV-2 variant XBB.1.16 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike XBB.1.16 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. meGFP-hTRAK1

    Plasmid

    #188664

    Purpose

    human TRAK1 with N-terminal meGFP tag

    Depositor

    Thomas Schwarz

    Insert

    OIP106 (TRAK1 Human)
    

    Use

    Tags

    meGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP_sfCherry2(11)_H2B

    Plasmid

    #82605

    Purpose

    Expresses sfCherry2(11) tagged H2B in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid

    #68375

    Purpose

    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK1

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #213480

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA-directcapture
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28b-irp3 WT

    Plasmid

    #87106

    Purpose

    expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

    Insert

    irp3 WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC1-dmito-oROS-HT

    Plasmid

    #216418

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    dmito-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD649-HAsp-IL11RA-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156788

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    IL11RA (IL11RA Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV 3xFLAG-LC3B WT

    Plasmid

    #123092

    Purpose

    Expresses 3xFLAG-LC3B wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge