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Organization of Chromatin by Intrinsic and Regulated Phase Separation.
Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry L, Gerlich DW, Redding S, Rosen MK
Cell. 2019 Oct 3;179(2):470-484.e21. doi: 10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
157792pAV5B+longBRD4Expression of long isoform of BRD4
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157793pETduet+p300HATExpression of p300 HAT domain
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157795pETduet+sfGFP-TetR-p300HATExpression of sfGFP-TetR-p300HAT fusion protein
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157796pMTTH+H1.4Expression of human linker histone H1.4
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157797pMTTH+H1.4ΔCTDExpression of human linker histone H1.4 without c-terminal tail
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157798pMTTH+LANA-H1.4CTDExpression of LANA peptide-human linker histone H1.4 CTD fusion protein
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157799pMTTH+mEGFP-H1.4Expression of mEGFP and human linker histone H1.4 fusion protein
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157800pMTTH+mEGFP-H1.4ΔCTDExpression of mEGFP fused to human linker histone H1.4 without c-terminal tail
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157801pMTTH+mEGFP-LANAExpression of mEGFP and LANA peptide fusion protein
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157802pMTTH+mEGFP-LANA-H1.4CTDExpression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein
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157803pMTTH+bromo5Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1
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157804pMTTH+eGFP-bromo5Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1
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157805pMTTH+eGFP-bromo1Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1
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157806pMTTH+eGFP-bromo5(N140A)Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation
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157807pET19b+H3Expression of cysteine-free human histone H3 (c111A)
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157808pET19b+H2B(T116C)Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry
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157809pET19b+H2BExpression of human histone H2B
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