Organization of Chromatin by Intrinsic and Regulated Phase Separation.
Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry L, Gerlich DW, Redding S, Rosen MK
Cell. 2019 Oct 3;179(2):470-484.e21. doi: 10.1016/j.cell.2019.08.037. Epub 2019 Sep 19. PubMed Article
Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry L, Gerlich DW, Redding S, Rosen MK
Cell. 2019 Oct 3;179(2):470-484.e21. doi: 10.1016/j.cell.2019.08.037. Epub 2019 Sep 19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157785 | p12x601 | EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone |
| 157786 | pWM_12x601_15bpLinker | EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes |
| 157787 | pWM_12x601_20bpLinker | EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes |
| 157788 | pWM_12x601_25bpLinker | EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes |
| 157789 | pWM_12x601_30bpLinker | EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes |
| 157790 | pWM_12x601_35bpLinker | EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes |
| 157791 | pWM_12x601_45bpLinker | EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes |
| 157792 | pAV5B+longBRD4 | Expression of long isoform of BRD4 |
| 157793 | pETduet+p300HAT | Expression of p300 HAT domain |
| 157794 | pETduet+sfGFP-TetR | Expression of sfGFP-TetR fusion protein |
| 157795 | pETduet+sfGFP-TetR-p300HAT | Expression of sfGFP-TetR-p300HAT fusion protein |
| 157796 | pMTTH+H1.4 | Expression of human linker histone H1.4 |
| 157797 | pMTTH+H1.4ΔCTD | Expression of human linker histone H1.4 without c-terminal tail |
| 157798 | pMTTH+LANA-H1.4CTD | Expression of LANA peptide-human linker histone H1.4 CTD fusion protein |
| 157799 | pMTTH+mEGFP-H1.4 | Expression of mEGFP and human linker histone H1.4 fusion protein |
| 157800 | pMTTH+mEGFP-H1.4ΔCTD | Expression of mEGFP fused to human linker histone H1.4 without c-terminal tail |
| 157801 | pMTTH+mEGFP-LANA | Expression of mEGFP and LANA peptide fusion protein |
| 157802 | pMTTH+mEGFP-LANA-H1.4CTD | Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein |
| 157804 | pMTTH+eGFP-bromo5 | Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 |
| 157805 | pMTTH+eGFP-bromo1 | Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1 |
| 157806 | pMTTH+eGFP-bromo5(N140A) | Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation |
| 157807 | pET19b+H3 | Expression of cysteine-free human histone H3 (c111A) |
| 157808 | pET19b+H2B(T116C) | Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry |
| 157809 | pET19b+H2B | Expression of human histone H2B |
| 157812 | pWM_12x601_193 NRL + TetO | EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes |