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Organization of Chromatin by Intrinsic and Regulated Phase Separation.
Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry L, Gerlich DW, Redding S, Rosen MK
Cell. 2019 Oct 3;179(2):470-484.e21. doi: 10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
157785p12x601EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone
157786pWM_12x601_15bpLinkerEcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes
157787pWM_12x601_20bpLinkerEcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes
157788pWM_12x601_25bpLinkerEcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes
157789pWM_12x601_30bpLinkerEcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes
157790pWM_12x601_35bpLinkerEcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes
157791pWM_12x601_45bpLinkerEcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes
157792pAV5B+longBRD4Expression of long isoform of BRD4
157793pETduet+p300HATExpression of p300 HAT domain
157794pETduet+sfGFP-TetRExpression of sfGFP-TetR fusion protein
157795pETduet+sfGFP-TetR-p300HATExpression of sfGFP-TetR-p300HAT fusion protein
157796pMTTH+H1.4Expression of human linker histone H1.4
157797pMTTH+H1.4ΔCTDExpression of human linker histone H1.4 without c-terminal tail
157798pMTTH+LANA-H1.4CTDExpression of LANA peptide-human linker histone H1.4 CTD fusion protein
157799pMTTH+mEGFP-H1.4Expression of mEGFP and human linker histone H1.4 fusion protein
157800pMTTH+mEGFP-H1.4ΔCTDExpression of mEGFP fused to human linker histone H1.4 without c-terminal tail
157801pMTTH+mEGFP-LANAExpression of mEGFP and LANA peptide fusion protein
157802pMTTH+mEGFP-LANA-H1.4CTDExpression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein
157803pMTTH+bromo5Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1
157804pMTTH+eGFP-bromo5Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1
157805pMTTH+eGFP-bromo1Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1
157806pMTTH+eGFP-bromo5(N140A)Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation
157807pET19b+H3Expression of cysteine-free human histone H3 (c111A)
157808pET19b+H2B(T116C)Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry
157809pET19b+H2BExpression of human histone H2B
157812pWM_12x601_193 NRL + TetOEcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Antibodies from Article