|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||130883||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Modifications to backboneremoved sgRNA expression cassette
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
/ Fusion Protein
- GFP (N terminal on insert)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GFP-RAB10 was a gift from Michael Bassik (Addgene plasmid # 130883 ; http://n2t.net/addgene:130883 ; RRID:Addgene_130883)
For your References section:Systematic identification of host cell regulators of Legionella pneumophila pathogenesis using a genome-wide CRISPR screen. Jeng EE, Varun Bhadkamkar, Nnejiuwa U. Ibe, Haley Gause, Lihua Jiang, Joanne Chan, Ruiqi Jian, David Jimenez-Morales, Erica Stevenson, Nevan J. Krogan, Danielle L. Swaney, Michael P. Snyder, Shaeri Mukherjee, Michael C. Bassik. Cell Host & Microbe, Sept 2019 10.1016/j.chom.2019.08.017