161,641 results

1. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

   Plasmid

   #231986

   Purpose

   Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   OVA-2A-ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pk7-GR-GFP

   Plasmid

   #15534

   Depositor

   Ian Macara

   Article

   Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The glucocorticoid receptor was cloned into the X…

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-X(4130)B1

   Plasmid

   #168018

   Purpose

   Expresses X(4130)B1-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   X(4130)B1-6xHis
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1delBRCT

   Plasmid

   #173938

   Purpose

   Bacterial expression of PARP1 lacking BRCT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delBRCT (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 374-520; V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. σ3-mCherry

   Plasmid

   #186580

   Purpose

   Expression of adaptor protein 3 (AP3) sigma subunit AP3S1 tagged with mCherry

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP3S1 (AP3S1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. UBC-4xmNG-dMCP

   Plasmid

   #231093

   Purpose

   Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).

   Depositor

   John Ngo

   Insert

   4xmNG-dMCP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSYC-187

   Plasmid

   #74794

   Purpose

   pCS4+-P2A-mCherry-CAAX

   Depositor

   Seok-Yong Choi

   Article

   Kim et al PLoS One. 2011 . 6(4):e18556.

   Insert

   P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1_SPTLC1_WT

   Plasmid

   #181920

   Purpose

   3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

   Depositor

   Christian Metallo

   Article

   Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

   Insert

   Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
   

   Use

   Lentiviral; Mammalian expression, , doxycycline i…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3β-FLAG-CBP-HA

   Plasmid

   #32908

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   CBP (Crebbp Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-huTsCas12a

    Plasmid

    #174687

    Purpose

    Expresses 6xHis-MBP tagged TsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huTsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IZ-ANXA11-mRuby3

    Plasmid

    #184894

    Purpose

    Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    R230C relative to NP_001148.1

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCVpuroATT

    Plasmid

    #47539

    Purpose

    Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

    Depositor

    David Weinstock

    Article

    Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pQE60-EFG-His

    Plasmid

    #124126

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor G (fusA Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. W118-1_hTGM1-Flag

    Plasmid

    #180406

    Purpose

    lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM1 (TGM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKL2351

    Plasmid

    #199721

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWM_12x601_20bpLinker

    Plasmid

    #157787

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_20bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-pelB

    Plasmid

    #157740

    Purpose

    empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Use

    Tags

    PelB signal sequence and possible His6-tag (adds …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiDP1.2

    Plasmid

    #171095

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Emerald, Geminin, miniAID, mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUAST-pHuji-GINKO2

    Plasmid

    #177117

    Purpose

    Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

    Depositor

    Robert Campbell

    Article

    Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

    Insert

    pHuji-GINKO2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70 promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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