159,237 results

1. pHsp83-NLS-HA-2xMCP-2xTagRFP-T

   Plasmid

   #71242

   Purpose

   Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   NLS-2xMCP-2xTagRFP-T
   

   Use

   P element-mediated germline transformation

   Tags

   TagRFP-T

   Expression

   Insect

   Mutation

   Promoter

   Hsp83

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-GFP-IKBalpha-wt

   Plasmid

   #15263

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS169

   Plasmid

   #48882

   Purpose

   Negative feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Insert

   lacI with ssrA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Para/lac

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJS167

   Plasmid

   #48881

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Inserts

   araC gene with ssrA tag
   monomeric yeast-enhanced green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Para/lac

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG1C

   Plasmid

   #71740

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDAS12489_PEAR-GFP_2in1_2.0

   Plasmid

   #177186

   Purpose

   PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Rab5A Q79L

   Plasmid

   #28046

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab5A (RAB5A Human)
   

   Use

   Tags

   EGFP on N-term

   Expression

   Mammalian

   Mutation

   Q79L

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRGEN-CMV-CjCas9

   Plasmid

   #89752

   Purpose

   Expression of CjCas9 in mammalian cells

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA HA HP1 beta

   Plasmid

   #24079

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 beta (CBX1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pGWB501

    Plasmid

    #74843

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pReporter_12

    Plasmid

    #60572

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TagRFP-T_A1aY1

    Plasmid

    #158751

    Purpose

    Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    TagRFP-T_A1aY1
    

    Use

    Lentiviral

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (CMV enhancer and chicken beta actin promoter)

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.2 TDP-43 NLS1 YFP

    Plasmid

    #84912

    Purpose

    Mammalian expresion of TDP-43 NLS1 YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A

    Promoter

    CMV

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Neo-Bam APC

    Plasmid

    #16507

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. p15A-OptoCre-knt-P**-R

    Plasmid

    #189851

    Purpose

    Cre-inducible activation of knt for kanamycin resistance.

    Depositor

    Mary Dunlop

    Article

    Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

    Insert

    P**-loxP-TT-loxP-R-knt
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P** (modified T7 phage gene 10)

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p15A-OptoCre-cat-P-R

    Plasmid

    #189853

    Purpose

    Cre-inducible activation of cat for chloramphenicol resistance.

    Depositor

    Mary Dunlop

    Article

    Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

    Insert

    P-loxP-TT-loxP-R-cat
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P (T7 phage gene 10)

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSW454 - pUFO

    Plasmid

    #115978

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pUFO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2246

    Plasmid

    #141989

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF12 (TCF12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-CRY2-iSH2

    Plasmid

    #66839

    Purpose

    Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    iSH2
    

    Use

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-hAgo2

    Plasmid

    #21981

    Depositor

    Phil Sharp

    Article

    Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge