-
PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 111765 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1(+)
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5399
- Total vector size (bp) 10166
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameClover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
-
Alt nameVinTS-tCRMod-GGSGGS9
-
SpeciesG. gallus (chicken), Synthetic
-
Insert Size (bp)4767
-
MutationResidues 1-228 of Clover, residues 1-3 deleted from mRuby2
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Partially based on Addgene Plasmids #26019, #49089, and #21769
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
VinTS-tCRMod-GGSGGS9 was a gift from Brenton Hoffman (Addgene plasmid # 111765 ; http://n2t.net/addgene:111765 ; RRID:Addgene_111765) -
For your References section:
Tunable molecular tension sensors reveal extension-based control of vinculin loading. LaCroix AS, Lynch AD, Berginski ME, Hoffman BD. Elife. 2018 Jul 19;7. pii: 33927. doi: 10.7554/eLife.33927. 10.7554/eLife.33927 PubMed 30024378