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pGART-GFP
(Plasmid #99106)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99106 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 9236
  • Modifications to backbone
    insertion of GFP from pLTG82 into XhoI and SalI restriction sites (SalI restriction site destroyed)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GART
  • Alt name
    TrifGART
  • Alt name
    phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3030
  • Mutation
    A65T, L68V, and A72S mutations in GFP gene; kanR/neoR selection markers destroyed (deletion of amino acids 138-144)
  • GenBank ID
    NM_000819
  • Entrez Gene
    GART (a.k.a. AIRS, GARS, GARTF, PAIS, PGFT, PRGS)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGART-GFP was a gift from Stephen Benkovic (Addgene plasmid # 99106 ; http://n2t.net/addgene:99106 ; RRID:Addgene_99106)
  • For your References section:

    Reversible compartmentalization of de novo purine biosynthetic complexes in living cells. An S, Kumar R, Sheets ED, Benkovic SJ. Science. 2008 Apr 4;320(5872):103-6. doi: 10.1126/science.1152241. 10.1126/science.1152241 PubMed 18388293