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PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

Plasmid

#168806

Purpose

PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ASCL1-P2A-DLX2-T2A-mCherry

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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mEos3.2-cortactin

Plasmid

#193287

Purpose

mammalian transient expression of mEos3.2-cortactin

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

cortactin (Cttn Mouse)

Tags

mEos3.2

Expression

Mammalian

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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eIF2a 3

Plasmid

#21809

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Mutation

S52D

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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SGLT1(GFP)-MAP17(nb)

Plasmid

#216210

Purpose

for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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GAL FLAG103QDProGFPp303

Plasmid

#15587

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLenti_rtTA-P2A-BFP_HygroR

Plasmid

#186738

Purpose

rtTA-P2A-BFP expression construct cloned into lentiviral backbone

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

rtTA

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6s

Plasmid

#80316

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6s

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-GAL4

Plasmid

#24345

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

GAL4

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-cMyc

Plasmid

#46970

Purpose

lentiviral expression of c-Myc

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1

Plasmid

#17791

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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tdTomato-pBAD

Plasmid

#54856

Purpose

Localization: Protein Expression Vector , Excitation: 554, Emission: 581

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

6xHis and tdTomato

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Mutation

deleted R4-R23 deleted exons 71-78

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-UbxD8

Plasmid

#53777

Purpose

express FLAG-tagged human UbxD8 gene

Depositor

Yihong Ye

Article

Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

Insert

UbxD8 (FAF2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only
PJ-INPP5E

Plasmid

#38001

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1 (SAC1 Budding Yeast)
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Mutation

Cys 779 mutated to Ser

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

Plasmid

#208977

Purpose

Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

human U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:GFP1–10

Plasmid

#125663

Purpose

Binary vector for the inducible expression of GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-BRD9

Plasmid

#65380

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD9 (BRD9 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD7

Plasmid

#195845

Purpose

Expresses human FZD7 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD7 (FZD7 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

Plasmid

#197061

Purpose

Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Inserts

Encapsulin
Adaptor Protein

Use

CRISPR

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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LeGO-G2

Plasmid

#25917

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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