159,258 results

1. SNAP-BAF

   Plasmid

   #227443

   Purpose

   Mammalian expression of SNAP-BAF

   Depositor

   David Pellman

   Article

   Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

   Insert

   Barrier-to-autointegration factor (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AfNitB_pET29b

   Plasmid

   #215401

   Purpose

   Expression construct for AfNitB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   AfNitB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MEK1-E102-I103del-V5

   Plasmid

   #217301

   Purpose

   Lentivirus vector for expression of MEK1 E102-I103deletion

   Depositor

   Channing Der

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   E102-I103deletion

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EXT1- D164A-pLJC2-3XFLAG

   Plasmid

   #215828

   Purpose

   Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
   

   Use

   Lentiviral

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

   Plasmid

   #211969

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

   Plasmid

   #211968

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p194-RV-CAG-dio-mScarlet-T2A-Brn2

   Plasmid

   #204741

   Purpose

   Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

   Depositor

   Lynette Lim

   Article

   Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

   Insert

   Brn2
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPPC005

   Plasmid

   #171140

   Purpose

   For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   XylS-Pm
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pm

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJT121_GalL_nCDA1-VQRBE3

   Plasmid

   #145086

   Purpose

   Expressing base editor nCDA1-VQRBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-VQRBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, D1135V, R1335Q, T1337R)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-ERBB2-D769H

    Plasmid

    #116327

    Purpose

    Lentiviral expression of ERBB2 D769H

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D769H

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-EPN51 (R40A)

    Plasmid

    #80109

    Purpose

    Late1-O3-33-myc-PH(R40A)

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-51 (R40A)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pRK5-myc-Miro1 E208K/E328K

    Plasmid

    #47894

    Purpose

    Expresses myc tagged Miro1 E208K/E328K mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro1 E208K/E328K (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    E208K/E328K, abolishes calcium binding

    Promoter

    CMV

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Sec61b-HA-mNG21-10

    Plasmid

    #141213

    Purpose

    Membrane Tethered Split Fluorescent Reporter for ER Retragrade

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    Sec61b-HA-mNG21-10
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI SMN2

    Plasmid

    #72287

    Purpose

    SMN2 exon 7 splicing cassette

    Depositor

    Elliot Androphy

    Article

    Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

    Insert

    SMN2 (SMN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains exons 6-7-8 including introns, multiple …

    Promoter

    CMV

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eforRed chromoprotein

    Plasmid

    #117837

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, eforRed
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV500 A-FOS

    Plasmid

    #33353

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    FOS (Fos Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative of c-Jun; heterodimerizes and i…

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-ESR1

    Plasmid

    #116737

    Purpose

    Lentiviral expression of ESR1

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-DIO-SYP-HRP

    Plasmid

    #117186

    Purpose

    Plasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge