165,688 results

1. pET14b – E. coli Exonuclease I

   Plasmid

   #104552

   Purpose

   bacterial expression of Exonuclease I

   Depositor

   Charles Bell

   Article

   Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

   Insert

   Exonuclease I (sbcB E.coli)
   

   Tags

   6X-His tag followed by thrombin site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

   Plasmid

   #184632

   Purpose

   Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gad67 (Gad1 Mouse)
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Rab11a-7

   Plasmid

   #55124

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab11a (RAB11A Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. EFSp-GFP-YAP (5SA)

   Plasmid

   #174170

   Purpose

   Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Five LATS phosphorylation sites (S61, S109, S127,…

   Promoter

   EFS

   Available Since

   Sept. 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLVX-IRES-Hygro_GCaMP6s

   Plasmid

   #164591

   Purpose

   Stable integration and expression of GCaMP6s in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-VP6SA11

   Plasmid

   #89166

   Purpose

   Express Rotavirus SA11 VP6 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP6
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTagRFP657-N1

   Plasmid

   #31959

   Depositor

   Vladislav Verkhusha

   Article

   Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

   Insert

   TagRFP657
   

   Expression

   Mammalian

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-NuMA

   Plasmid

   #81029

   Purpose

   Expresses human NuMA with N-terminal EGFP tag in mammalian cells

   Depositor

   Michael Mancini

   Insert

   Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Promoter

   EF1a

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-ET

    Plasmid

    #72542

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa)

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP SNAP47

    Plasmid

    #101915

    Purpose

    Expresses SNAP47 in mammalian cells

    Depositor

    Thierry Galli

    Article

    Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

    Insert

    SNAP47 (SNAP47 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-DIO-Ef1a-TVA-2A-N2cG

    Plasmid

    #172360

    Purpose

    Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRISPR-SB

    Plasmid

    #177936

    Purpose

    Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP rbFOX2

    Plasmid

    #63086

    Purpose

    Mammalian expression of EGFP tagged FOX2

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    RBFOX2 (RBFOX2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRU55

    Plasmid

    #167684

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCpf1
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid

    #137830

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Leucine 71 to Lysine in CTEVp fragment

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPing

    Plasmid

    #47100

    Purpose

    Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.

    Depositor

    Wayne Parrott

    Article

    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

    Inserts

    Ping cDNA
    mPing
    hygromycin resistance gene
    

    Use

    Plant expression

    Tags

    GFP

    Expression

    Yeast

    Promoter

    CaMV35s and StUbi3

    Available Since

    Sept. 16, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. MD-2

    Plasmid

    #20864

    Depositor

    Jie-Oh Lee

    Article

    Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

    Insert

    Myeloid differentiation factor 2 (LY96 Human)
    

    Tags

    protein A tag

    Expression

    Insect

    Mutation

    contains amino acids 19–160

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR4-HaloTag (w876-1)

    Plasmid

    #29644

    Depositor

    Eric Campeau

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HaloTag

    Available Since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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