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Depositing Lab
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Depositing OrganizationCalifornia Institute of Technology
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 32678-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 32678-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEYFP-C1
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Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 4684
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Modifications to backboneYFP was replaced with GFP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec24D
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Alt nameSEC24 family, member D
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SpeciesH. sapiens (human)
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Insert Size (bp)3168
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GenBank IDAF130464
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Entrez GeneSEC24D (a.k.a. CLCRP2)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe original pEYFP-Sec24D construct was provided by Dr. David Stephens, Professor of Cell Biology, at the University of Bristol.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 32678-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 32678-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-Sec24D was a gift from Henry Lester (Addgene plasmid # 32678 ; http://n2t.net/addgene:32678 ; RRID:Addgene_32678) -
For your References section:
Trafficking of {alpha}4* Nicotinic Receptors Revealed by Superecliptic Phluorin: EFFECTS OF A {beta}4 AMYOTROPHIC LATERAL SCLEROSIS-ASSOCIATED MUTATION AND CHRONIC EXPOSURE TO NICOTINE. Richards CI, Srinivasan R, Xiao C, Mackey ED, Miwa JM, Lester HA. J Biol Chem. 2011 Sep 9;286(36):31241-9. Epub 2011 Jul 18. 10.1074/jbc.M111.256024 PubMed 21768117
