163,517 results

1. CamKII-LGI1-pHmScarlet

   Plasmid

   #185538

   Purpose

   Expresses LGI1-pHmScarlet under the CamKII promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   LGI1 (Lgi1 Rat)
   

   Use

   Lentiviral

   Tags

   pHmScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiFC-bFosVC155

   Plasmid

   #22013

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   bFosVC155
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   Oct. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEmerald-Annexin-12

   Plasmid

   #53991

   Purpose

   Localization: Plasma Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Annexin (ANXA4 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pMuLE Lenti Dest iRFP

   Plasmid

   #62176

   Purpose

   MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Lentiviral; Mule gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. nontarget S. pyogenes scaffold CARGO array

   Plasmid

   #191319

   Purpose

   Contains an array of nontargeting guide RNAs

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL2-eNOSPromoter-Luciferase

   Plasmid

   #19297

   Depositor

   Charles Lowenstein

   Article

   Arnet et al J Biol Chem. 1996 Jun 21. 271(25):15069-

   Insert

   endothelial nitric oxide synthase promoter (NOS3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pUA66 theta GFP

   Plasmid

   #105607

   Purpose

   Expresses GFP with no promoter

   Depositor

   Pamela Silver

   Article

   Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   No Promoter

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lck-mTurquoise2

   Plasmid

   #98822

   Purpose

   In vivo visualization of the plasma membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Lck
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mScarlet-MYO10

   Plasmid

   #145179

   Purpose

   Expresses mScarlet-MYO10 in mammalian cells

   Depositor

   Johanna Ivaska

   Article

   Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

   Insert

   mScarlet-MYO10 (MYO10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9D10A_GFP

    Plasmid

    #44720

    Purpose

    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9D10A-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    D10A mutation- nickase mutant

    Promoter

    CAG

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBA407

    Plasmid

    #85970

    Purpose

    UPRE-mCh-Ubc-Neo

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    mCherry
    Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-Flag-hsDicer

    Plasmid

    #41584

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-myc3-CUL4A

    Plasmid

    #19951

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CUL4A (cullin 4A) (CUL4A Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Rab27B

    Plasmid

    #89447

    Purpose

    Expression of GFP-tagged human Rab27B in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B (RAB27B Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

    Plasmid

    #67979

    Purpose

    Cas9 activity reporter (control) with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBad-CA

    Plasmid

    #105665

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO (pEHA1339)

    Plasmid

    #209088

    Purpose

    Backbone plasmid for Tet Responsive transgene expression, FRT integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJAM1.19_PB-CIT-PGK-Blast

    Plasmid

    #205564

    Purpose

    Express human CIT

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    CIT (CIT Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163076

    Purpose

    Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSET-4xTR-TUBE

    Plasmid

    #110312

    Purpose

    E. coli expression and purification of 4xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    4xTR-TUBE (UBQLN1 Human)
    

    Use

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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