159,258 results

1. pcDNA3.1-Tat101(AD8)-Flag

   Plasmid

   #115777

   Purpose

   pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

   Depositor

   Damian Purcell

   Article

   Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

   Insert

   HIV-1 Tat protein (AD8)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-TEV-EGFP-PRC1

   Plasmid

   #167540

   Purpose

   For expression of His6-TEV-EGFP-PRC1 in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

   Insert

   PRC1 (PRC1 Human)
   

   Use

   Tags

   EGFP and His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

   Plasmid

   #130976

   Purpose

   Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   Hook3-SNAPf (HOOK3 Human)
   

   Use

   Tags

   8His-ZZ-LTLT and SNAPf

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. TGN38 EGFP

   Plasmid

   #128148

   Purpose

   Expression of TGN38 amino acids 304-357

   Depositor

   Jennifer Lippincott-Schwartz

   Insert

   TGN38 (Tgoln2 Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Contains amino acids 304-357

   Promoter

   Available since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-HA-Ubiquitin-K6

   Plasmid

   #22900

   Depositor

   Sandra Weller

   Article

   Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K6 only, all other lysines mutated to arginines

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. PA-mCherry1-Fascin-C-10

   Plasmid

   #57177

   Purpose

   Localization: Cytoskeleton, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Fascin (FSCN1 Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-fLuc=tdT

   Plasmid

   #120870

   Purpose

   Piggybac transposon plasmid for live animal optical imaging

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   pcDNA3-fLuc=tdT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LifeAct-mGarnet2

   Plasmid

   #104308

   Purpose

   codes for a fusion of fluorescent protein mGarnet2 and lifeact that targets F-actin in live cells

   Depositor

   Ulrich Nienhaus

   Article

   Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

   Insert

   LifeAct-mGarnet2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 HA-SUMO2-Q90P

   Plasmid

   #36448

   Purpose

   Expresses HA-Sumo2Q90P mutant in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutated Q90P

   Promoter

   T7

   Available since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCAX DAB1

    Plasmid

    #30139

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Disabled-1 (Dab1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pAPM-D4 miR30-TRIM28 ts1

    Plasmid

    #115862

    Purpose

    TRIM28 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    TRIM28 shRNA (TRIM28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabePuro-bcl2

    Plasmid

    #21144

    Depositor

    Junying Yuan

    Article

    Miura et al Cell. 1993 Nov 19. 75(4):653-60.

    Insert

    pBabePuro-bcl 2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF0086

    Plasmid

    #141510

    Purpose

    Lentiviral vector for overexpressing the LIN28B transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LIN28B (LIN28B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myc-Nix

    Plasmid

    #100795

    Purpose

    Mammalian expression of Nix (Bnip3L)

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-GFP11-pDEST-N

    Plasmid

    #118366

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    GFP11-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMIH-TFAM-GFP

    Plasmid

    #113704

    Purpose

    Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    TFAM (Tfam Mouse)
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH-EF1-GFP

    Plasmid

    #133018

    Purpose

    (Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

    Depositor

    Michael Huen

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TERT - WT

    Plasmid

    #213827

    Purpose

    Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    human TERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. NPY-mCherry

    Plasmid

    #67156

    Purpose

    Expresses NPY-mCherry

    Depositor

    Wolfhard Almers

    Article

    Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

    Insert

    NPYmCherry (NPY Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pScaffold-H1 donor

    Plasmid

    #118152

    Purpose

    PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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