pCMV-QF
(Plasmid #24339)

• Depositing Lab: Liqun Luo
• Publication: Potter et al Volume 141, Issue 3, 536-548, 30 April 2010

Backbone

• Vector backbone: pcDNA3.1-Myc-His-A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5493
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: QF
• Species: Neurospora crassa
• Insert Size (bp): 2450
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Reference:
  • pCMV-QF (GenBank)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Obtained by subcloning QF cDNA using BamHI and Not I from pAC-QF into pCDNA3.1-
myc-His-A.

A stop codon precedes the Myc and His tags.