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Depositing Lab
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Depositing OrganizationUniversity of Alabama at Birmingham
Located in United States of America -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 32362-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 32362-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMS2
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Vector typeMycobacterial GFP expression vector
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegfpm2+
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Alt namecodon optimized GFP for mycobacteria
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer psmyc1
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).
Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554
DNA (Catalog # 32362-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 32362-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMN437 was a gift from Michael Niederweis (Addgene plasmid # 32362 ; http://n2t.net/addgene:32362 ; RRID:Addgene_32362) -
For your References section:
Identification of outer membrane proteins of Mycobacterium tuberculosis. Song H, Sandie R, Wang Y, Andrade-Navarro MA, Niederweis M. Tuberculosis (Edinb). 2008 Nov;88(6):526-44. Epub 2008 Apr 25. 10.1016/j.tube.2008.02.004 PubMed 18439872
Map uploaded by the depositor.
