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pML1342
(Plasmid #32376)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32376 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    int L5; xylEm; ttsbiA, ttsbiB

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This expression vector integrates at the mycobacteriophage L5 site and can be stabilized by excision of the vector backbone including the int gene using the Cre recombinase; contains xylE gene as a reporter for integration; terminators to protect expression cassette against transcriptional interference.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML1342 was a gift from Michael Niederweis (Addgene plasmid # 32376 ; http://n2t.net/addgene:32376 ; RRID:Addgene_32376)
  • For your References section:

    Taking phage integration to the next level as a genetic tool for mycobacteria. Huff J, Czyz A, Landick R, Niederweis M. Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6. 10.1016/j.gene.2010.07.012 PubMed 20692326