Purposeyeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49455||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGüldener U., et al. (1996)
- Backbone size w/o insert (bp) 7854
Vector typeYeast Expression, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameCre-EBD fusion
- Promoter Gal1 promoter
- Cloning method Unknown
- 5′ sequencing primer Gal1 primer
- 3′ sequencing primer Cyc1 primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid pSH62-EBD was generated by an in vivo recombination approach.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSH62-EBD was a gift from Marc Gartenberg (Addgene plasmid # 49455 ; http://n2t.net/addgene:49455 ; RRID:Addgene_49455)
For your References section:Controlling gene expression in yeast by inducible site-specific recombination. Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR. Nucleic Acids Res. 2000 Dec 15;28(24):E108. 10.1093/nar/28.24.e108 PubMed 11121495