|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11256||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5043
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
p4339 + pRS410 --> pRS418. Insert is NatMX (clonNAT resistance) from EcoRI-cut p4339. Backbone is NcoI-cut pRS410 (CEN plasmid).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS418 was a gift from Fred Cross (Addgene plasmid # 11256 ; http://n2t.net/addgene:11256 ; RRID:Addgene_11256)