|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11259||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6098
Vector typeYeast Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
pRS400 + pRS425 --> pRS420. Insert is KanMX (G418 resistance) from AatII-cut pRS400. Backbone is ApaLI-cut pRS425 (2 micron plasmid).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS420 was a gift from Fred Cross (Addgene plasmid # 11259 ; http://n2t.net/addgene:11259 ; RRID:Addgene_11259)