Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes.
Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O
Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773. PubMed Article
Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O
Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48203 | Twitch-2B pRSETB | Fluorescent reporter for calcium signaling |
| 48204 | Twitch-3 pRSETB | Fluorescent reporter for calcium signaling |
| 49531 | Twitch-2B pcDNA3 | Fluorescent reporter for calcium signaling |
| 49532 | Twitch-3 pcDNA3 | Fluorescent reporter for calcium signaling |
| 49533 | Twitch-4 pcDNA3 | Fluorescent reporter for calcium signaling |
| 49812 | Twitch-1 pcDNA3 | Fluorescent reporter for calcium signaling |
| 100039 | pAAV.CAG.Flex.Twitch2B.WPRE.SV40 | AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling |
| 100040 | pAAV.hSyn1.Twitch2B.WPRE.SV40 | AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling |
| 104500 | Twitch-2C pRSETB | fluorescent reporter for calcium signaling |
| 104501 | Twitch-2C pcDNA3 | fluorescent reporter for calcium signaling |