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(Plasmid #51300)


Item Catalog # Description Quantity Price (USD)
Plasmid 51300 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pET SUMO
  • Backbone manufacturer
  • Backbone size (bp) 5643
  • Modifications to backbone
    1) MultiCloning Site (MCS) of pET28b vector inserted in the pET SUMO cloning site 2) Addition of 2 codon for His residues to the 6 already present in the original vector 3) Removal of the original EcoRI site located inside SUMO ORF 4) Removal of the original HindIII site
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tag / Fusion Protein
    • His8 + SUMO (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector is a modified version of the commercial (sell as linearized) pET SUMO vector of Invitrogen. This vector has been optimized for restriction enzyme cloning, thanks to the inserted MultiCloning Site of pET28b.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIOX was a gift from Andrea Mattevi (Addgene plasmid # 51300 ; ; RRID:Addgene_51300)