MAVS-Pex
(Plasmid #44557)

• Purpose: Retroviral vector for expression of a chimeric MAVS allele targeted to peroxisomes in mammalian cells
• Depositing Lab: Jonathan Kagan
• Publication: Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 2010 May 6.

Backbone

• Vector backbone: pMSCV2.2 IRES GFP
• Backbone size w/o insert (bp): 6600
• Total vector size (bp): 8400
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAVS-Pex13 chimeric protein
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Mutation: contains aa1-500 of MAVS and aa136-233 of Pex13
• GenBank ID: BC044952 and AAH44952.1 for MAVS. NM_002618 and NP_002609.1 for Pex13
• Entrez Gene: MAVS (a.k.a. CARDIF, IPS-1, IPS1, VISA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pMSCV forward
• 3′ sequencing primer: MAVS scn

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ZJ Chen provided HA-MAVS in pEF
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry