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Purpose(Empty Backbone) for ectopic constitutive expression of two mycobacterial proteins in one vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepVV16, pSE100, pMC1m
- Backbone size (bp) 4961
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Vector typeBacterial Expression ; E.coli-mycobacteria shuttle vector
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Tags
/ Fusion Proteins
- His tag (N terminal on backbone)
- Flag tag (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pST-2K was a gift from Vinay Nandicoori (Addgene plasmid # 44564 ; http://n2t.net/addgene:44564 ; RRID:Addgene_44564) -
For your References section:
Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria. Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, Nandicoori VK. Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 10.1128/AEM.03695-12. Epub 2013 Jan 11. 10.1128/AEM.03695-12 PubMed 23315736
Map uploaded by the depositor.
