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NPY-pHTomato
(Plasmid #83501)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83501 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPY
  • Alt name
    neuropeptide-Y
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Promoter CMV
  • Tag / Fusion Protein
    • pHTomato (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pHTomato was provided from Yulong Li (School of Life Sciences, Peking University, Life Sciences Building, Mailbox 142, 5 Yi He Yuan Road, Haidian, Beijing, China, 100871) and Tsien 2012, Nature Neuroscience 15, 1047–1053.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NPY-pHTomato was a gift from Sebastian Barg (Addgene plasmid # 83501 ; http://n2t.net/addgene:83501 ; RRID:Addgene_83501)
  • For your References section:

    Survey of Red Fluorescence Proteins as Markers for Secretory Granule Exocytosis. Gandasi NR, Vesto K, Helou M, Yin P, Saras J, Barg S. PLoS One. 2015 Jun 19;10(6):e0127801. doi: 10.1371/journal.pone.0127801. eCollection 2015. PONE-D-15-03957 [pii] PubMed 26091288