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NPY-pHTomato
(Plasmid #83501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83501 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 83501-D50 50 μg of DNA in Tris buffer $495
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DNA 83501-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPY
  • Alt name
    neuropeptide-Y
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Entrez Gene
    NPY (a.k.a. PYY4)
  • Promoter CMV
  • Tag / Fusion Protein
    • pHTomato (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pHTomato was provided from Yulong Li (School of Life Sciences, Peking University, Life Sciences Building, Mailbox 142, 5 Yi He Yuan Road, Haidian, Beijing, China, 100871) and Tsien 2012, Nature Neuroscience 15, 1047–1053.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NPY-pHTomato was a gift from Sebastian Barg (Addgene plasmid # 83501 ; http://n2t.net/addgene:83501 ; RRID:Addgene_83501)

  • For your References section:

    Survey of Red Fluorescence Proteins as Markers for Secretory Granule Exocytosis. Gandasi NR, Vesto K, Helou M, Yin P, Saras J, Barg S. PLoS One. 2015 Jun 19;10(6):e0127801. doi: 10.1371/journal.pone.0127801. eCollection 2015. PONE-D-15-03957 [pii] PubMed 26091288
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