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pCDNAHygro(+)LAMP2B
(Plasmid #86029)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86029 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNAHygro(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 6257
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lysosome-associated membrane protein 2b
  • Alt name
    LAMP2b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    657
  • Entrez Gene
    LAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (unknown if destroyed)
  • 3′ cloning site Apa1 (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNAHygro(+)LAMP2B was a gift from Janice Blum (Addgene plasmid # 86029 ; http://n2t.net/addgene:86029 ; RRID:Addgene_86029)
  • For your References section:

    Lamp-2a facilitates MHC class II presentation of cytoplasmic antigens. Zhou D, Li P, Lin Y, Lott JM, Hislop AD, Canaday DH, Brutkiewicz RR, Blum JS. Immunity. 2005 May;22(5):571-81. 10.1016/j.immuni.2005.03.009 PubMed 15894275