pcDNAZeo(-) LAMP2C
(Plasmid #89342)

• Purpose: Expresses human LAMP-2C in mammalian cells
• Depositing Lab: Janice Blum
• Publication: Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. doi: 10.4049/jimmunol.1501476. Epub 2016 Feb 8.

Backbone

• Vector backbone: pcDNA3.1/Zeo(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6236
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lysosomal-associated membrane protein 2C
• Alt name: LAMP-2C
• Alt name: LAMP2C
• Species: H. sapiens (human)
• Insert Size (bp): 1236
• GenBank ID: AY561849.1
• Entrez Gene: LAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry