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MSCV-(mcs) human CSF1 full length-IRES-Puro
(Plasmid #86797)


Item Catalog # Description Quantity Price (USD)
Plasmid 86797 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    macrophage colony stimulating factor 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CSF1 (a.k.a. CSF-1, MCSF, PG-M-CSF)
  • Tag / Fusion Protein
    • IRES-Puro (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN5; pBABE5
  • 3′ sequencing primer pCDH-rev
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The mcs is from the pBabe vector that was inserted into the MSCV vector to provide more cloning sites, and was developed by Dr Den Besten. The human CSF-1 cDNAs were cloned by the Cetus Company.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full length CSF-1 is secreted

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-(mcs) human CSF1 full length-IRES-Puro was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 86797 ; ; RRID:Addgene_86797)
  • For your References section:

    Transforming potential of the c-fms proto-oncogene (CSF-1 receptor). Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ. Nature. 1987 Feb 5-11;325(6104):549-52. 10.1038/325549a0 PubMed 3027579