165,688 results

1. TFORF0985

   Plasmid

   #142249

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

   Plasmid

   #192204

   Purpose

   Lenti-GL(GFP-Luciferase)

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-GL(GFP-Luciferase)
   

   Use

   Lentiviral; Mammalian expression

   Mutation

   NA

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. YL-CashGem-BAT1-URA3-LINEAR

   Plasmid

   #174837

   Purpose

   pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   GPDp

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Roxed-Cre

   Plasmid

   #51273

   Purpose

   Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   Roxed-Cre
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-TetO-Klf5

   Plasmid

   #128830

   Purpose

   dox-dependent expression of mouse Klf5

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Klf5 (Klf5 Mouse)
   

   Use

   Lentiviral

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSilencer-Opa1 KO

   Plasmid

   #191949

   Purpose

   Expression of a siRNA that targets Opa1

   Depositor

   Luca Scorrano

   Insert

   Opa1
   

   Expression

   Mammalian

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

   Plasmid

   #184632

   Purpose

   Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gad67 (Gad1 Mouse)
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EFS-foldon-GFP- PCP

   Plasmid

   #194503

   Purpose

   Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

   Depositor

   Chunqing Song

   Article

   Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

   Insert

   foldon-GFP-PCP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1α core promoter

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-VP3SA11

   Plasmid

   #89164

   Purpose

   Express Rotavirus SA11 VP3 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP3
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

    Plasmid

    #204359

    Purpose

    AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDq-mCherry-P2A-HA-KORD
    

    Use

    AAV

    Tags

    HA (for KORD) and mCherry (for miniDq)

    Mutation

    For miniDq, the third intracellular loop (ICL3) o…

    Promoter

    hSyn

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbA5c-MevT(CO)-MBIS(CO, ispA)

    Plasmid

    #35151

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5,

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a hMDH2

    Plasmid

    #204230

    Purpose

    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH2 (MDH2 Human)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Does not include transit peptide and includes a M…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_STAT1_WT

    Plasmid

    #82234

    Purpose

    Gateway Donor vector containing STAT1, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-puro/Flag PD-L1 N35/3NQ

    Plasmid

    #121455

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N192Q, N200Q and N219Q

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbS8a-RFP

    Plasmid

    #35274

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid

    #139992

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.4_17G6-1 light chain

    Plasmid

    #198250

    Purpose

    recombinant expression of light chain of monoclonal anti-AMP antibody 17G6-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    light chain of monoclonal anti-AMP antibody 17G6-1 (mouse kappa)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Expression

    Mammalian

    Mutation

    * see comments

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87828

    Purpose

    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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