163,517 results

1. pX330A_dCas9-1x4

   Plasmid

   #63598

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS2273

   Plasmid

   #111899

   Purpose

   ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple343 (TUBB3 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ple343 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH-AFID-3

   Plasmid

   #167361

   Purpose

   For inducing predictable deletions in rice plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD_dGFPmut3-AAV

   Plasmid

   #187379

   Purpose

   arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

   Depositor

   Roi Avraham

   Insert

   dGFPmut3_AAV
   

   Use

   Tags

   AAV

   Expression

   Bacterial

   Mutation

   C-terminal AAV tail

   Promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3TS-NLS-TurboID

   Plasmid

   #163849

   Purpose

   In vitro transcription of nls-TurboID. Parton lab clone JST

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID
   

   Use

   In vitro transcription of rna

   Tags

   nls

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST2T18ms

   Plasmid

   #72647

   Purpose

   Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

   Depositor

   Brian Kvitko

   Type

   Empty backbone

   Use

   Sucrose counter-selection allelic exchange vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. mRNA-CleanCap-mNeonGreen

   Plasmid

   #188248

   Purpose

   Template plasmid to transcribe mRNA encoding mNeonGreen

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC2HB

   Plasmid

   #63734

   Purpose

   bait plasmid for an adapted two-hybrid system for C. albicans

   Depositor

   Patrick Van Dijck

   Article

   Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

   Type

   Empty backbone

   Use

   C. albicans bait plasmid

   Tags

   HA, LexA, and NLS

   Expression

   Mutation

   Promoter

   Met3

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tag5Amyc-GSK3b WT

   Plasmid

   #16260

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   GSK3 beta (GSK3B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK-caCas9-NatMX-Neut5L-Leu2 drive

    Plasmid

    #89578

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Leu2 gene drive
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-pDisplay-SNAP

    Plasmid

    #223495

    Purpose

    Expression of SNAP-tags to outer surface in mammalian cells

    Depositor

    James Frank

    Article

    Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

    Insert

    SNAP-tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast HA-GPAT4

    Plasmid

    #173168

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EphA2-MT

    Plasmid

    #108851

    Purpose

    Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

    Depositor

    Kalina Hristova

    Article

    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

    Insert

    EPHA2 (EPHA2 Human)
    

    Use

    Tags

    Labeled with mTurquoise on the C-Terminus via a 1…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLENTI_hACE2_PURO

    Plasmid

    #155295

    Purpose

    Lentiviral vector to generate hACE2 stable expressing cell line

    Depositor

    Raffaele De Francesco

    Insert

    hACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    C9

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. TH

    Plasmid

    #39079

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TH (TH Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. mTagBFP2-TFR-20

    Plasmid

    #55327

    Purpose

    Localization: Transferin Receptor, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    TFR (TFRC Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3.1/CALR wt-FLAG

    Plasmid

    #204532

    Purpose

    Mammalian expression of human CALR wt-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR wt-FLAG (CALR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p189

    Plasmid

    #65207

    Purpose

    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMSCVFLAG-ZFP423

    Plasmid

    #24764

    Depositor

    Bruce Spiegelman

    Article

    Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

    Insert

    Zinc Finger Protein 423 (Zfp423 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2010

    Availability

    Academic Institutions and Nonprofits only

20. Mito-mEosEM

    Plasmid

    #132706

    Purpose

    application in Correlative light-electron microscopy

    Depositor

    Pingyong Xu

    Article

    Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    mitochondria targeting sequence
    

    Use

    Tags

    mEosEM

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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