161,570 results

1. pcDNA3β-FLAG-CBP-HA

   Plasmid

   #32908

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   CBP (Crebbp Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC019 - LwCas13a from Leptotrichia wadei

   Plasmid

   #91909

   Purpose

   Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a and guide expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Lenti_MCP-p300_Hygro

   Plasmid

   #138459

   Purpose

   lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   p300 core
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT85_SpdCas9_pmcDA1

   Plasmid

   #204614

   Purpose

   CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FKBP-GFP-Sec61β

   Plasmid

   #172442

   Purpose

   Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Protein transport protein Sec61 subunit beta
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1 gfaABC1D-Ezr-BioID2-HA

   Plasmid

   #176744

   Purpose

   Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Ezr-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTOX1

   Plasmid

   #127446

   Purpose

   Encodes YhaV toxin. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Insert

   yhaV toxin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRha

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSADdeltaG-F3

   Plasmid

   #32634

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   B19N, B19P, B19M and B19L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   In frame insertion of Ser after aa10 in M (matrix…

   Promoter

   CMV

   Available since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRN111

   Plasmid

   #84463

   Purpose

   pJB38-NWMN29-30 + SarA_P1-mCherry-Term

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   SarA_P1-mCherry from pRN11
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-FOXO3a TM

    Plasmid

    #1788

    Depositor

    Michael Greenberg

    Article

    Brunet et al Cell 1999 Mar 19;96(6):857-68.

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A (no longer detectably phosphor…

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. mNeptune2-C1

    Plasmid

    #54836

    Purpose

    Localization: C1 Cloning Vector, Excitation: 600, Emission: 650

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mNeptune2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pEGFP-N1 hMOG-alpha1-EGFP

    Plasmid

    #126463

    Purpose

    Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Myelin oligodendrocyte glycoprotein (MOG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GPR161-Tango

    Plasmid

    #66334

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR161 (GPR161 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCytERM_mScarlet_N1

    Plasmid

    #85066

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(-)-qmTQ2-ATP

    Plasmid

    #225248

    Purpose

    mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1.D2-NP

    Plasmid

    #134366

    Purpose

    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    D2-NP (DRD2 Human)
    

    Use

    Tags

    Flag, natural peptide of nanoluciferase, and sign…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Circular-ic-CVB3-GFP

    Plasmid

    #226260

    Purpose

    Production of in situ circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Minos ITR loxp MCS lox2272

    Plasmid

    #218983

    Purpose

    Plasmid containing ITR sequences compatible with Minos transposase and multiple cloning site

    Depositor

    Amro Hamdoun

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-StrepII

    Plasmid

    #208617

    Purpose

    Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Strep II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge